Anti-Lactate Dehydrogenase Isoenzyme V抗体(ab9002)

概述

  • 产品名称Anti-Lactate Dehydrogenase Isoenzyme V抗体
    参阅全部 Lactate Dehydrogenase Isoenzyme V 一抗
  • 描述
    羊多克隆抗体to Lactate Dehydrogenase Isoenzyme V
  • 特异性Identity has been confirmed by double diffusion (Ouchterlony) against purified LDH V and a known anti-human LDH V. Specificity has been demonstrated by Western blot against Liver Cell Lysate. We have never performed a cross-reactivity check to LDH I, II, III, or IV, however, due to the similarity between LDH V and LDH's II, III and IV there is a possibility that there will be some cross-reactivity.
  • 经测试应用适用于: IHC-P, WB, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human Lactate Dehydrogenase V, purified from human placenta.

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: Human Kidney Tissue; Human Liver Tissue; HEK293 Whole Cell; HepG2 Whole Cell.

性能

应用

Our Abpromise guarantee covers the use of ab9002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent dilution. PubMed: 12942121
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IHC-Fr 1/100. PubMed: 21569415Snap freeze tissue in liquid nitrogen and store. Then the sections should be fixed for 10 minutes in acetone and rehydrated in PBS.

靶标

  • 相关性Lactate dehydrogenase 5 (LDH5) is one of five LDH isoenzymes and important for promoting anaerobic glycolysis. LDH5 is transcriptionally regulated by the hypoxia inducible factors (HIF) 1a and 2a.
  • 细胞定位Cytoplasmic
  • 数据库链接
  • 别名
    • Cell proliferation-inducing gene 19 protein antibody
    • L lactate dehydrogenase A chain antibody
    • Lactate dehydrogenase A antibody
    • LDH A antibody
    • LDH M antibody
    • LDH muscle subunit antibody
    • LDH1 antibody
    • LDH5 antibody
    • LDHA antibody
    • PIG 19 antibody
    • PIG19 antibody
    • Renal carcinoma antigen NY-REN-59 antibody
    see all

Anti-Lactate Dehydrogenase Isoenzyme V antibody 图像

  • All lanes : Anti-Lactate Dehydrogenase Isoenzyme V antibody (ab9002) at 1 µg/ml

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : Human liver tissue lysate - total protein (ab29889)
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Rabbit polyclonal Secondary Antibody to Sheep IgG - H&L (HRP) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 36 kDa


    Exposure time : 30 seconds
  • Immunohistochemical analysis of Human head and neck cancer tissue, staining Lactate Dehydrogenase Isoenzyme V (red) with ab9002 at 1/100 dilution. Sections were fixed in acetone before being incubated with primary antibody overnight at 4°C. A Cy3-conjugated donkey anti-sheep IgG (1/600) was used as the secondary antibody.

Anti-Lactate Dehydrogenase Isoenzyme V antibody (ab9002)参考文献

This product has been referenced in:
  • Wojtkowiak JW  et al. Pyruvate sensitizes pancreatic tumors to hypoxia-activated prodrug TH-302. Cancer Metab 3:2 (2015). IHC-P ; Mouse . Read more (PubMed: 25635223) »
  • Koukourakis MI  et al. Lactate dehydrogenase 5 isoenzyme overexpression defines resistance of prostate cancer to radiotherapy. Br J Cancer 110:2217-23 (2014). WB ; Human . Read more (PubMed: 24714743) »

See all 11 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Bladder Tumor Cell Line)
Permeabilization Yes - 01% v/v Triton X-100
Specification Bladder Tumor Cell Line
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Dr. Dimitra Kalamida

Verified customer

提交于 Sep 12 2016

Application Western blot
Sample Human Cell lysate - whole cell (prostate cancer cell line)
Gel Running Conditions Reduced Denaturing (10%acrylamide)
Loading amount 20 µg
Specification prostate cancer cell line
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Stamatia Pouliliou

Verified customer

提交于 Jun 26 2015

Application Western blot
Sample Human Cell lysate - whole cell (Epithelial cells)
Gel Running Conditions Non-reduced Denaturing (10)
Loading amount 30 µg
Specification Epithelial cells
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Achilleas Mitrakas

Verified customer

提交于 Jun 15 2015

Thank you for your phone call and your interest in our products.
As discussed over the phone, the Anti-Lactate Dehydrogenase Isoenzyme V antibody (ab9002) has been used by several research groups in performing immunohistochemical staining. Having r...

Read More

Thank you for your phone call.

DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab9002 in mouse. This code will give you: 1 free PRIMARY ANTIBODY before the expirati...

Read More

Thank you for contacting Abcam. I’ve had a look into this and done a bit of a web search and as far as I can see LDH5, LDHM and LDHA are all different names for the same protein, as stated in this paper, http://www.pnas.org/content/107/5/2037.long....

Read More

From our testing we would expect our anti LDH-V to show a single band of approximately 36 kDa by Western blot - this band corresponds to the A subunit (sometimes called the muscle subunit) which LDH-V is entirely composed of.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"