RabMAb

Anti-Lactate Dehydrogenase C抗体[EP1746Y] (ab52747)

概述

  • 产品名称Anti-Lactate Dehydrogenase C抗体[EP1746Y]
    参阅全部 Lactate Dehydrogenase C 一抗
  • 描述
    兔单克隆抗体[EP1746Y] to Lactate Dehydrogenase C
  • 特异性This antibody reacts with Lactate Dehydrogenase C. The immunogen sequence is 100% identical to Lactate Dehydrogenase C, but there is the possibility of cross-reactivity for lactate dehydrogenase A and lactate dehydrogenase B at 69% and 82%, respectively.
  • 经测试应用适用于: WB, Flow Cyt, IHC-Pmore details
    不适用于: ICC or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Lactate Dehydrogenase C aa 300 to the C-terminus (C terminal).
    Database link: P07864

  • 阳性对照
    • WB: Human testis lysate. IHC-P: Human testis carcinoma tissue.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab52747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/20000. Detects a band of approximately 31 kDa (predicted molecular weight: 36 kDa).
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.
  • 应用说明Is unsuitable for ICC or IP.
  • 靶标

    • 功能Possible role in sperm motility.
    • 通路Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
    • 序列相似性Belongs to the LDH/MDH superfamily. LDH family.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Cancer/testis antigen 32 antibody
      • CT32 antibody
      • EC 1.1.1.27 antibody
      • L lactate dehydrogenase C chain antibody
      • L-lactate dehydrogenase C chain antibody
      • Lactate dehydrogenase c variant 1 antibody
      • Lactate dehydrogenase c variant 3 antibody
      • Lactate dehydrogenase c variant 4 antibody
      • Lactate dehydrogenase C4 antibody
      • LDH C antibody
      • LDH testis subunit antibody
      • LDH X antibody
      • LDH-C antibody
      • LDH-X antibody
      • LDH3 antibody
      • ldhc antibody
      • LDHC_HUMAN antibody
      • LDHX antibody
      • MGC111073 antibody
      see all

    Anti-Lactate Dehydrogenase C antibody [EP1746Y] 图像

    • Anti-Lactate Dehydrogenase C antibody [EP1746Y] (ab52747) at 1/20000 dilution + Human testis lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 36 kDa
      Observed band size : 31 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical staining of paraffin-embedded human testis carcinoma using ab52747 at a 1/50 dilution.

    Anti-Lactate Dehydrogenase C antibody [EP1746Y] (ab52747)参考文献

    This product has been referenced in:
    • Rolland AD  et al. Identification of genital tract markers in the human seminal plasma using an integrative genomics approach. Hum Reprod 28:199-209 (2013). Read more (PubMed: 23024119) »
    • Wyns C  et al. Long-term spermatogonial survival in cryopreserved and xenografted immature human testicular tissue. Hum Reprod 23:2402-14 (2008). IHC-P ; Human . Read more (PubMed: 18664476) »

    See all 2 Publications for this product

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