Anti-Lactate Dehydrogenase B抗体[60H11] (ab85319)


  • 产品名称Anti-Lactate Dehydrogenase B抗体[60H11]
    参阅全部 Lactate Dehydrogenase B 一抗
  • 描述
    小鼠单克隆抗体[60H11] to Lactate Dehydrogenase B
  • 经测试应用适用于: ICC/IF, Flow Cyt, WB, IP, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    His tagged recombinant Human lactate dehydrogenase B protein, purified from E.coli.

  • 阳性对照
    • HeLa, RD, Ramos, A431, Jurkat, K562 and C6 cell lysates.



Our Abpromise guarantee covers the use of ab85319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB 1/5000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
IP Use at an assay dependent concentration. Use 5µl.
ELISA 1/5000 - 1/10000.
IHC-P Use a concentration of 5 µg/ml.


  • 通路Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
  • 疾病相关Note=Defects in LDHB result in deficiency of lactate dehydrogenase, a condition with no clear symptomatic consequences. Lactate dehydrogenase deficiency can probably be considered a non-disease.
  • 序列相似性Belongs to the LDH/MDH superfamily. LDH family.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Epididymis secretory protein Li 281 antibody
    • HEL S 281 antibody
    • L lactate dehydrogenase B chain antibody
    • L-lactate dehydrogenase B chain antibody
    • Lactate dehydrogenase H chain antibody
    • LDH B antibody
    • LDH H antibody
    • LDH heart subunit antibody
    • LDH-B antibody
    • LDH-H antibody
    • LDHB antibody
    • LDHB_HUMAN antibody
    • LDHBD antibody
    • LDHH antibody
    • Renal carcinoma antigen NY REN 46 antibody
    • Renal carcinoma antigen NY-REN-46 antibody
    • TRG-5 antibody
    • TRG5 antibody
    see all

Anti-Lactate Dehydrogenase B antibody [60H11] 图像

  • All lanes : Anti-Lactate Dehydrogenase B antibody [60H11] (ab85319) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : RD cell lysate
    Lane 3 : Ramos cell lysate
    Lane 4 : A431 cell lysate
    Lane 5 : Jurkat cell lysate
    Lane 6 : K562 cell lysate
    Lane 7 : C6 cell lysate

    Predicted band size : 37 kDa
    Observed band size : 37 kDa
  • ICC/IF image of ab85319 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85319, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab85319 staining in Human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85319, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Overlay histogram showing HeLa cells stained with ab85319 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85319, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Anti-Lactate Dehydrogenase B antibody [60H11] (ab85319) at 1/5000 dilution + Natural human Lactate Dehydrogenase B protein (ab78521) at 0.1 µg

    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds
  • lactate dehydrogenase B was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to Lactate Dehydrogenase B and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab85319.
    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
    Band: 37kDa: lactate dehydrogenase B

Anti-Lactate Dehydrogenase B antibody [60H11] (ab85319)参考文献

This product has been referenced in:
  • Ding F  et al. Ovariectomy induces a shift in fuel availability and metabolism in the hippocampus of the female transgenic model of familial Alzheimer's. PLoS One 8:e59825 (2013). WB ; Mouse . Read more (PubMed: 23555795) »
  • Billiard J  et al. Quinoline 3-sulfonamides inhibit lactate dehydrogenase A and reverse aerobic glycolysis in cancer cells. Cancer Metab 1:19 (2013). Read more (PubMed: 24280423) »

See all 2 Publications for this product

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Thank you for contacting us.

We are sorry. We have not measured a concentration of this particular antibody.

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The antibody ab85319, anti-LDHb, reacts with western blots of recombinant LDHb but not blots containing recombinant LDHa. Please let us know if you have any questions.

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Thanks for your enquiry.

I have requested any further information from the lab regarding testing of ab85319 for reactivity with LDHa.

Also, please note that ab4710 is not an antibody product but instead is a tissue slide. I believe ...

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Thank you for your reply and for sending the image again with your protocol information. There does seem to be a lot of background on the blot, but this can be reduced by further diluting the antibody (we recommend 1:5000-1:10000). Once the background ...

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Thank you for contacting us with your question. I could not find the image attached to your email- could you please resend it? I also have a few questions so that I can better help you: 1) Do you have your original order number ...

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