Anti-Lactate Dehydrogenase LDHA + LDHB抗体[10E6AA9] (ab112996)
Key features and details
- Mouse monoclonal [10E6AA9] to LDHA + LDHB
- Suitable for: ICC/IF, IP, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Lactate Dehydrogenase LDHA + LDHB抗体[10E6AA9] -
描述
小鼠单克隆抗体[10E6AA9] to LDHA + LDHB -
宿主
Mouse -
特异性
Shotgun immunization of human HeLa cell lysates into mice. Targets were determined by mass spectrometry and validated by WB, ICC, ELISA pair and other techniques. -
经测试应用
适用于: ICC/IF, IP, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa cells and cell lysate.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99.98% HEPES buffered saline -
Concentration information loading...
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纯度
Proprietary Purification -
纯化说明
ab112996 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Monoclonal purity was near homogeneity as judged by SDS-PAGE (>95%). -
克隆
单克隆 -
克隆编号
10E6AA9 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab112996于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 2 µg/ml.
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IP |
Use at an assay dependent concentration.
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Flow Cyt |
Use a concentration of 1 - 2 µg/ml.
Triton permeabilization required. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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ICC/IF
Use a concentration of 2 µg/ml. |
IP
Use at an assay dependent concentration. |
Flow Cyt
Use a concentration of 1 - 2 µg/ml. Triton permeabilization required. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
图片
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ab112996 at 2ug/ml staining Lactate Dehydrogenase B/LDH-B in HeLa cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). The target protein locates mainly to the cytoplasm.
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ab112996 at 1ug/ml staining Lactate Dehydrogenase B/LDH-B in Triton X-100 permeabilized HeLa cells by flow cytometry (blue). Isotype control antibody (red).
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The 36.6 kDa LDHB protein along with 36.7kDa LDHA protein were immunoprecipitated from Hela cell lysate with ab112996. The identities of the proteins were confirmed by mass spectrometry. This gel was stained with colloidal Coomassie blue G.
实验方案
数据表及文件
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Datasheet download
文献 (1)
ab112996 被引用在 1 文献中.
- Ran F et al. miR-1224-3p Promotes Breast Cancer Cell Proliferation and Migration through PGM5-Mediated Aerobic Glycolysis. J Oncol 2021:5529770 (2021). PubMed: 33986801