Anti-Lactate Dehydrogenase B抗体[10E6AA9] (ab112996)

概述

  • 产品名称Anti-Lactate Dehydrogenase B抗体[10E6AA9]
    参阅全部 Lactate Dehydrogenase B 一抗
  • 描述
    小鼠单克隆抗体[10E6AA9] to Lactate Dehydrogenase B
  • 特异性Shotgun immunization of human HeLa cell lysates into mice. Targets were determined by mass spectrometry and validated by WB, ICC, ELISA pair and other techniques.
  • 经测试应用适用于: ICC/IF, IP, In-Cell ELISA, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Hela cell cytosol fraction

  • 阳性对照
    • HeLa cells and cell lysate.
  • 常规说明

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

Our Abpromise guarantee covers the use of ab112996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 2 µg/ml.
IP Use at an assay dependent concentration.
In-Cell ELISA Use a concentration of 2 µg/ml.
Flow Cyt Use a concentration of 1 - 2 µg/ml. Triton permeabilization required.



ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 通路Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
  • 疾病相关Note=Defects in LDHB result in deficiency of lactate dehydrogenase, a condition with no clear symptomatic consequences. Lactate dehydrogenase deficiency can probably be considered a non-disease.
  • 序列相似性Belongs to the LDH/MDH superfamily. LDH family.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Epididymis secretory protein Li 281 antibody
    • HEL S 281 antibody
    • L lactate dehydrogenase B chain antibody
    • L-lactate dehydrogenase B chain antibody
    • Lactate dehydrogenase H chain antibody
    • LDH B antibody
    • LDH H antibody
    • LDH heart subunit antibody
    • LDH-B antibody
    • LDH-H antibody
    • LDHB antibody
    • LDHB_HUMAN antibody
    • LDHBD antibody
    • LDHH antibody
    • Renal carcinoma antigen NY REN 46 antibody
    • Renal carcinoma antigen NY-REN-46 antibody
    • TRG-5 antibody
    • TRG5 antibody
    see all

Anti-Lactate Dehydrogenase B antibody [10E6AA9] 图像

  • ab112996 at 2ug/ml staining Lactate Dehydrogenase B in HeLa cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). The target protein locates mainly to the cytoplasm.
  • ab112996 at 1ug/ml staining Lactate Dehydrogenase B in Triton X-100 permeabilized HeLa cells by flow cytometry (blue). Isotype control antibody (red).
  • Isolation of LDHB using ab112996 by immunoprecipitation.
    The identity of the protein was confirmed by mass spectrometry. The gel was stained with colloidal Coomassie blue G.

Anti-Lactate Dehydrogenase B antibody [10E6AA9] (ab112996)参考文献

ab112996 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab112996.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"