Anti-Lactate Dehydrogenase抗体(Biotin) (ab7638)

概述

  • 产品名称Anti-Lactate Dehydrogenase抗体(Biotin)
    参阅全部 Lactate Dehydrogenase 一抗
  • 描述
    山羊多克隆抗体to Lactate Dehydrogenase (Biotin)
  • 偶联物Biotin
  • 经测试应用适用于: ELISA, Immunomicroscopy, WB, Dot blotmore details
  • 种属反应性
    与反应: Mouse, Rabbit, Human, Chinese Hamster
    预测可用于: a wide range of other species
  • 免疫原

    Full length native Lactate Dehydrogenase (purified).

  • 阳性对照
    • purified enzyme from rabbit muscle(Worthington Biochemicals)
  • 常规说明

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule.

性能

应用

Our Abpromise guarantee covers the use of ab7638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
Immunomicroscopy
WB
Dot blot
  • 应用说明Dot: Use at an assay dependent dilution.
    ELISA: 1/4000 - 1/20000.
    IM: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Please note: The non-conjugated version of this antibody (ab2101) has been used in WB in the reference Zaman K et al (protocol available on the datasheet for ab2101).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 通路Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
    • 疾病相关Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
    • 序列相似性Belongs to the LDH/MDH superfamily. LDH family.
    • 翻译后修饰ISGylated.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Cell proliferation-inducing gene 19 protein antibody
      • GSD11 antibody
      • L lactate dehydrogenase B chain antibody
      • L-lactate dehydrogenase A chain antibody
      • Lactate dehydrogenase A antibody
      • Lactate dehydrogenase B antibody
      • Lactate dehydrogenase H chain antibody
      • Lactate dehydrogenase M antibody
      • LDH A antibody
      • LDH B antibody
      • LDH H antibody
      • LDH heart subunit antibody
      • LDH M antibody
      • LDH muscle subunit antibody
      • LDH-A antibody
      • LDH-M antibody
      • LDH1 antibody
      • ldha antibody
      • LDHA_HUMAN antibody
      • LDHBD antibody
      • LDHM antibody
      • MS1111 antibody
      • PIG19 antibody
      • Proliferation inducing gene 19 antibody
      • Renal carcinoma antigen NY REN 46 antibody
      • Renal carcinoma antigen NY-REN-59 antibody
      • TRG 5 antibody
      • TRG5 antibody
      see all

    Anti-Lactate Dehydrogenase antibody (Biotin) (ab7638)参考文献

    This product has been referenced in:
    • Rudyk O  et al. Protein kinase G oxidation is a major cause of injury during sepsis. Proc Natl Acad Sci U S A 110:9909-13 (2013). Mouse . Read more (PubMed: 23716652) »
    • Jerde TJ  et al. STRETCH INDUCTION OF CYCLOOXYGENASE-2 EXPRESSION IN HUMAN UROTHELIAL CELLS IS CALCIUM AND PROTEIN KINASE C ZETA-DEPENDENT. Mol Pharmacol : (2007). WB ; Human . Read more (PubMed: 17925460) »

    See all 3 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application ELISA
    Sample Chinese Hamster Cell (Ovary)
    Specification Ovary
    Type Direct
    Username

    Abcam user community

    Verified customer

    提交于 Apr 11 2012

    I am very pleased to hear the customer would like to accept our offer and test ab7638 in Chinese hamster.

    DISCOUNT CODE: #####
    Expiration date: ######

    This code will give you 1 free primary antibodybefore the expiration date....

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    Thank you for your enquiry.

    I am sorry to confirm that as far as we are aware, ab7638 Lactate Dehydrogenase antibody (Biotin)] has never been tested with samples from Chinese hamster. All tested and guaranteed species cross-reactivity inform...

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    Unfortunately, this information is not known.

    The unconjugated form (ab2101) was raised against rabbit LDH. This is expected to react with other species, although this has not been tested yet.

    ab7638 is linked to biotin. For Western blotting procedures such as this we would recommend ab2101 followed by a secondary against goat linked to HRP (such as ab7125).

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"