Anti-Lactate Dehydrogenase抗体[AF14A11] (ab85326)


  • 产品名称Anti-Lactate Dehydrogenase抗体[AF14A11]
    参阅全部 Lactate Dehydrogenase 一抗
  • 描述
    小鼠单克隆抗体[AF14A11] to Lactate Dehydrogenase
  • 经测试应用适用于: Flow Cyt, WB, IP, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    His tagged recombinant Human Lactate Dehydrogenase protein purified from E.coli.

  • 阳性对照
    • HeLa, MCF7, HepG2, 293T, SK-N-MC, RD and IMR32 cell lysates.



Our Abpromise guarantee covers the use of ab85326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/100. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB 1/2000 - 1/10000. Detects a band of approximately 30 kDa.
IP Use at an assay dependent concentration. Use 0.5µg
ELISA 1/2000 - 1/10000.


Anti-Lactate Dehydrogenase antibody [AF14A11] 图像

  • All lanes : Anti-Lactate Dehydrogenase antibody [AF14A11] (ab85326) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : 293T cell lysate
    Lane 5 : SK-N-MC cell lysate
    Lane 6 : RD cell lysate
    Lane 7 : IMR32 cell lysate

    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing HeLa cells stained with ab85326 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85326, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Lactate Dehydrogenase antibody [AF14A11] (ab85326)参考文献

This product has been referenced in:
  • Tseng CY & Firestein BL The Role of PSD-95 and Cypin in Morphological Changes in Dendrites Following Sublethal NMDA Exposure. J Neurosci 31:15468-80 (2011). WB ; Rat . Read more (PubMed: 22031893) »

See 1 Publication for this product

Product Wall

ab85326 was raised against recombinant human lactate dehydrogenase, not a synthetic peptide, and the antibody has not been epitope mapped.
The antibody is specific to LDHA, which is found predominantly in muscle tissue.
We have tested the cross...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HCT116)
Loading amount 10 µg
Specification HCT116
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5%

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提交于 Oct 13 2010