概述

  • 产品名称Anti-L3MBTL1抗体
    参阅全部 L3MBTL1 一抗
  • 描述
    兔多克隆抗体to L3MBTL1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Dog
  • 免疫原

    Synthetic peptide within residues: RRREGHGTDS EMGQGPVRES QSSDPPALQF RISEYKPLNM AGVEQPPSPE , corresponding to N terminal amino acids 2-51 of Human L3MBTL1 (NP_056293)

  • 阳性对照
    • Human fetal brain lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab85463 is purified by a peptide affinity chromatography method.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab85463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Polycomb group (PcG) protein that specifically recognizes and binds mono- and dimethyllysine residues on target proteins, therey acting as a 'reader' of a network of post-translational modifications. PcG proteins maintain the transcriptionally repressive state of genes: acts as a chromatin compaction factor by recognizing and binding mono- and dimethylated histone H1b/HIST1H1E at 'Lys-26' (H1bK26me1 and H1bK26me2) and histone H4 at 'Lys-20' (H4K20me1 and H4K20me2), leading to condense chromatin and repress transcription. Recognizes and binds p53/TP53 monomethylated at 'Lys-382', leading to repress p53/TP53-target genes. Also recognizes and binds RB1/RB monomethylated at 'Lys-860'. Participates in the ETV6-mediated repression. Probably plays a role in cell proliferation. Overexpression induces multinucleated cells, suggesting that it is required to accomplish normal mitosis.
  • 组织特异性Widely expressed. Expression is reduced in colorectal cancer cell line SW480 and promyelocytic leukemia cell line HL-60.
  • 序列相似性Contains 1 C2HC-type zinc finger.
    Contains 3 MBT repeats.
    Contains 1 SAM (sterile alpha motif) domain.
  • 发展阶段In interphase cells, it is scattered throughout the nucleoplasm. In mitotic cells, it strongly associates with condensed chromosomes from the prophase to telophase.
  • 结构域The MBT repeat 2 specifically recognizes and binds monomethylated and dimethylated proteins. In contrast, it does not bind trimethylated proteins. The MBT repeat 1 does not bind methylated peptides but inserts a proline ring in a Pro-Ser-Ser/Thr sequence context.
  • 细胞定位Nucleus. Excluded from the nucleolus. Does not colocalizes with the PcG protein BMI1, suggesting that these two proteins do not belong to the same complex.
  • Information by UniProt
  • 数据库链接
  • 别名
    • dJ138B7.3 antibody
    • H-L(3)MBT antibody
    • H-l(3)mbt protein antibody
    • KIAA0681 antibody
    • L(3)mbt protein homolog antibody
    • L(3)mbt-like antibody
    • L3MBT antibody
    • L3MBTL antibody
    • L3MBTL1 antibody
    • Lethal (3) malignant brain tumor l(3 antibody
    • Lethal(3)malignant brain tumor-like protein 1 antibody
    • LMBL1_HUMAN antibody
    • RGD1307316 antibody
    • ZC2HC3 antibody
    see all

Anti-L3MBTL1 antibody 图像

  • Anti-L3MBTL1 antibody (ab85463) at 1 µg/ml + Human fetal brain lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 88 kDa
    Observed band size : 88 kDa

Anti-L3MBTL1 antibody (ab85463)参考文献

ab85463 has not yet been referenced specifically in any publications.

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