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Tissue, cells or virus corresponding to Human L1CAM. Homogenous suspension of 16 week human foetal brain.
Database link: P32004
Our Abpromise guarantee covers the use of ab20148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 200-220 kDa. It may also detect smaller cleavage fragments (please see Notes below).|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
Ab20148 Immunoprecipitating L1CAM in human Melanoma whole cell lysate. 1000 µg of cell lysate was incubated with primary antibody (5 µg/mg lysate, ab20148) and matrix (Protein G) for 12 hours at 4°C. For western blotting ab20148 (1/5000) was used to confirm successful immunoprecipitation.