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chicken NgCAM protein (ab24345 detects the C-terminus portion of the protein that is conserved with mammalian L1)
Our Abpromise guarantee covers the use of ab24345 in the following tested applications.
|ICC/IF||1/300 - 1/1000.|
|IHC-FoFr||1/300 - 1/1000.|
|Flow Cyt||1/20. (paraformaldehyde or methanol fixed cells)
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||1/1000. Detects a band of approximately 200 kDa. Cleavage products observed at 60-80 kDa.|
This image is courtesy of Martin Grumet, Rutgers University, United States
ab24345 recognizes one or two polypeptides of L1 or Ng-CAM corresponding to the full length protein (~200kDa) as well as 60-80 kDa C-terminal cleavage products (as shown in the figure).
ab24345 (1/500) immunostaining L1CAM in 4% PFA-fixed rat cerebellum tissue. ML = molecular layer and FT = fiber tract of the cerebellum.