概述

  • 产品名称Anti-l + S Afadin抗体
    参阅全部 l + S Afadin 一抗
  • 描述
    兔多克隆抗体to l + S Afadin
  • 特异性Anti l/s Afadin specifically recognizes l afadin and s afadin by immunoblotting (approximately 200 and 190 kDa respectively). Staining of the l/s afadin bands is specifically inhibited by the immunizing peptide.
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog, Human
  • 免疫原

    Synthetic peptide:

    CKLPGDDRLMKNRADHRS

    conjugated to maleimide-activated BSA, corresponding to amino acids 1163-1179 of Rat l + S Afadin (with N-terminal added cysteine).

  • 常规说明Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

性能

应用

Our Abpromise guarantee covers the use of ab11338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 200 (l), 190 (s) kDa.
ICC/IF Use at an assay dependent dilution.

靶标

  • 功能Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton.
  • 疾病相关Note=A chromosomal aberration involving MLLT4 is associated with acute leukemias. Translocation t(6;11)(q27;q23) with MLL/HRX. The result is a rogue activator protein.
  • 序列相似性Contains 1 dilute domain.
    Contains 1 FHA domain.
    Contains 1 PDZ (DHR) domain.
    Contains 2 Ras-associating domains.
  • 结构域The PDZ/DHR domain interacts with the C-terminus of nectin and the Pro-rich C-terminus domain interacts with F-actin.
  • 细胞定位Cell junction > adherens junction. Not found at cell-matrix AJs.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AF6 antibody
    • AFAD_HUMAN antibody
    • Afadin antibody
    • ALL1-fused gene from chromosome 6 protein antibody
    • Mllt4 antibody
    • Protein Af-6 antibody
    • Protein AF6 antibody
    see all

Anti-l + S Afadin antibody (ab11338)参考文献

This product has been referenced in:
  • Lachke SA  et al. The cell adhesion gene PVRL3 is associated with congenital ocular defects. Hum Genet : (2011). IHC-Fr ; Mouse . Read more (PubMed: 21769484) »
  • Sasaki AT  et al. Ubiquitination of K-Ras enhances activation and facilitates binding to select downstream effectors. Sci Signal 4:ra13 (2011). Read more (PubMed: 21386094) »

See all 2 Publications for this product

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