Anti-l-Myc抗体(ab28739)

概述

  • 产品名称Anti-l-Myc抗体
    参阅全部 l-Myc 一抗
  • 描述
    兔多克隆抗体to l-Myc
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    A region within synthetic peptide: LERAVSDRLA PGAPRGNPPK ASAAPDCTPS LEAGNPAPAA PCPLGEPKTQ, corresponding to internal sequence amino acids 105-154 of Human l-Myc

  • 阳性对照
    • Human foetal kidney lysate, human kidney tissue.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab28739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 2.5 µg/ml. Predicted molecular weight: 40,25 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-l-Myc antibody 图像

  • Immunohistochemical staining of paraffin embedded human kidney with ab28739 at 4-8 ug/ml. Arrows indicate postive staining of epithelial cells of the renal tube.
  • Anti-l-Myc antibody (ab28739) at 2.5 µg/ml + Human foetal kidney lysate

    Secondary
    HRP conjugated anti-Rabbit IgG 1: 50,000 - 100,000

    Predicted band size : 40,25 kDa
    Observed band size : 23 kDa (why is the actual band size different from the predicted?)

Anti-l-Myc antibody (ab28739)参考文献

ab28739 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply. For the c-myc antibody, I suggest that you look at ab56 or ab11917. A positive control would be ab7914. Please let me know which would be best for you. I also hope that you are able to find the replacement l-myc that was...

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Thank you for contacting Abcam.

I am sorry to hear of the continued disappointing results from ab28739. I would be glad to send you a different antibody against l-myc or to issue you a credit note for the purchase. Please let me know what you...

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To my knowledge, customized protocols were not used in for this product. I've linked our standard western blotting protocol here: http://www.abcam.com/index.html?pageconfig=resource&rid=13045 In our published sample, Anti-l-Myc antibody (ab28739)was us...

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Thank you for contacting us regarding this product. I am sorry that you have been experiencing difficulties using our product and look forward to hearing the results of your positive control experiment. At Abcam, providing quality service is just as...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293 cell)
Loading amount 50 µg
Specification HEK293 cell
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Abcam user community

Verified customer

提交于 Aug 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Renal Pelvis of transgenic mouse)
Specification Renal Pelvis of transgenic mouse
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Nov 24 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"