L-Lactate Assay试剂盒(Colorimetric/Fluorometric) (ab65330)


  • 产品名称L-Lactate Assay试剂盒(Colorimetric/Fluorometric)
    参阅全部 L-Lactate 试剂盒
  • 测试
    100 x 1 assay
  • 样品类型
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids
  • 检测类型Quantitative
  • 灵敏度
    > 0.001 mM
  • 范围
    0.001 mM - 10 mM
  • 检测时间
    0h 40m
  • 种属反应性
    与反应: all Mammals
  • 产品概述

    L-Lactate Assay Kit (Colorimetric/Fluorometric) (ab65330) is an assay where lactate specifically reacts with a enzyme mix to generate a product, which interacts with lactate probe to produce color (570 nm) and fluorescence (at Ex/Em = 535/587 nm). The kit provides a convenient means for detecting L(+)-Lactate in biological samples such as in blood circulation, in cells, in culture mediums, in fermentation mediums, etc. There is no need of pretreatment or purification of samples.
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    L(+)-Lactate is the major stereo-isomer of lactate formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present but only at about 1-5% of the concentration of L(+)-Lactate.

  • 经测试应用适用于: Functional Studiesmore details


  • 存放说明Store at -20°C. Please refer to protocols.
  • 组件 标识符 100 tests
    L(+)-Lactate Standard (100 nmol/µl) Yellow 1 x 100µl
    Lactate Assay Buffer WM 1 x 25ml
    Lactate Enzyme Mix (lyophilized) Green 1 vial
    Lactate Probe (in DMSO, anhydrous) Red 1 x 200µl
  • 研究领域
  • 相关性Lactate (CH3CH(OH)COO-) plays important roles in many biological processes. Abnormal high concentration of lactate has been related to disease states such as diabetes and lactate acidosis, etc. L(+)-Lactate is the major stereoisomer of lactate formed in human intermediary metabolism and is present in blood. The lactate to pyruvate ratio reflects the redox state of the cell and describes the balance beween NAD+ and NADH, which is dependent on the interconversion of lactate and pyruvate via lactate dehydrogenase (LDH).


Our Abpromise guarantee covers the use of ab65330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

L-Lactate Assay Kit (Colorimetric/Fluorometric) 图像

  • Relative signal (RFU) in unfiltered human plasma (dilution 1:200) and mouse urine (dilution 1:200), comparing L-lactate signals with background readings ((-); no enzyme) after 10 minutes of incubation (duplicates +/- SD).

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Lactate measured in cell culture supernatants showing quantity (µmol) per mL of tested sample. Samples were diluted 40-160 fold and measured fluorometrically.

  • Lactate measured in biological fluids showing quantity (µmol) per mL of tested sample. Samples were diluted 10-40 fold and measured fluorometrically.


L-Lactate Assay Kit (Colorimetric/Fluorometric) (ab65330)参考文献

This product has been referenced in:
  • Howe S  et al. Lactate as a novel quantitative measure of viability in Schistosoma mansoni drug sensitivity assays. Antimicrob Agents Chemother 59:1193-9 (2015). Read more (PubMed: 25487803) »
  • Matos P  et al. A role for the mitochondrial-associated protein p32 in regulation of trophoblast proliferation. Mol Hum Reprod 20:745-55 (2014). Read more (PubMed: 24874554) »

See all 8 Publications for this product

Product Wall

I can confirm that the assay buffer and the standard need to be stored at -20C as stated on the datasheet.

However, if assays will be run in small batches, we can recommend aliquoting these components and storing them to prevent free...

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Yes, our ab65330 L-Lactate Assay Kit (Fluorometric) can be used for colorimetric detection. The difference this kit has with the ab65331 L-Lactate Assay Kit (Colorimetric) is indeed mainly the probe, but the detection limits are also different for...

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I can confirm that we suggest to start with 10mg of tissue (muscle for example) or 10 million cells if using single cells.

This will vary widely depending on the tissue or cell type.

If you are using a cell type where the ...

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I cannot give the exact compositions of the enzyme mixes in these kits, but the probes are just pure probes in a solvent. They do not have any other components. The enzyme mixes are obviously a combination of multiple enzymes. It does not have any flor...

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Thank you for contacting us. It should have mentioned in the protocol the incubation time is 30 minutes. We will be sure to correct the protocol.

I hope this information is helpful to you. Please do not hesitate to contact us if you need ...

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Thank you for contacting Abcam.
Since the lactate dehydrogenase (LDH) in tissue lysates degrades lactate, be sure to keep your samples at -80ºC. Below is our recommended homogenization protocol:
1. Homogenize the tissue samples (should contain ...

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Thank you for your enquiry.

We would recommend to use the assay buffer provided with ab65330 L-Lactate Assay Kit (Fluorometric) for cells. This buffer contains some proprietary ingredients which are crucial for the working efficiency of this...

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Thank you for contacting Abcam earlier today.

To prepare an intracellular lysate to use in the assay, we recommend using at least 2 x 10^6 cells. Then homogenize the cells in the assay buffer that is provided and centrifuge it gently to get ri...

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Gracias por contactarnos.

El kit de detección de Lactato debe mantenerse a -20C. Almacenado a esta temperatura y evitando ciclos de congelación y descongelación, el kit dura hasta la fecha indicada en la caja del kit, que...

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Thank you for contacting us.

I am not familiar with the contents of MRSB media, but in general cell culture supernatants will be compatible with these kits. If the media contains serum, we recommend deproteinizing the samples using our 10 kDa...

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