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L-Lactate Assay Kit (Colorimetric) (ab65331) is an assay where lactate is oxidized by lactate dehydrogenase to generate a product which interacts with a probe to produce a color (?max = 450 nm). The kit detects L(+)-Lactate in biological samples such as serum or plasma, cells, culture and fermentation media. There is no need for pretreatment or purification of samples.
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L(+)-Lactate is the major stereo-isomer of lactate formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present but only at about 1-5% of the concentration of L(+)-Lactate.
|L(+)-Lactate Standard (100 nmol/µl)||Yellow||1 x 100µl|
|Lactate Assay Buffer||WM||1 x 25ml|
|Lactate Enzyme Mix (lyophilized)||Green||1 vial|
|Lactate Substrate Mix||Red||1 vial|
Our Abpromise guarantee covers the use of ab65331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Linearity of dilution: concentration of L-Lactate in differently diluted (X-axis) biological samples, demonstrating a linearity of 89%-111% (concentrations corrected for by factor of dilution; duplicates; +/- SD).
Relative signal (RFU) in unfiltered human plasma (dilution 1:8), comparing L-lactate signals with background reading (no enzyme) after 10 minutes of incubation (duplicates +/- SD).
Standard curve with background signal subtracted (duplicates; +/- SD).