Anti-L Glutamate抗体(ab62668)

概述

  • 产品名称Anti-L Glutamate抗体
    参阅全部 L Glutamate 一抗
  • 描述
    鸡多克隆抗体to L Glutamate
  • 特异性No measurable cross-reactivity (<1:1000) against glutamate in peptides or proteins. Fixed tissue cross-reactivity tested with known targets at recommended dilution. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) against L-alanine,gamma-aminobutyrate, D/L-aspartate,1-amino-4-guanidobutane (AGB), D/L-arginine, L-citrulline, L-cysteine, D/L-glutamine, glutathione, glycine, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine. Modest cross-reactivity (1:20) against D-glutamate. Significant cross-reactivity (1:8) against free NAAG in competition assays (NAAG is not retained in aldehyde-fixed tissue).
  • 经测试应用适用于: IHC - Wholemount, ICCmore details
  • 种属反应性
    与反应: Species independent
  • 免疫原

    L Glutamate cross-linked to purifed fraction V bovine serum albumin with glutaraldehyde.

性能

应用

Our Abpromise guarantee covers the use of ab62668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC - Wholemount Use at an assay dependent concentration.
ICC 1/100. Endogenous content mapping by LM and EM immunocytochemistry. True dilution at user dilution: 1/32000. Optimal fixation: 0.1-2.5% glutaraldehyde,1% formaldehyde using HPI (High Performance Immunocytochemistry). Minimum glutaraldehyde: 0.0% using EHPI (Enhanced HPI) with 4% formaldehyde.

靶标

  • 相关性L Glutamate is a non-essential amino acid naturally occurring in the L-form. It is the most common excitatory neurotransmitter in the Central Nervous System.
  • 别名
    • Glutamate antibody
    • L glutamate antibody

Anti-L Glutamate antibody 图像

  • IHC - Wholemount of mouse brain tissue labelling L Glutamate with ab62668. Sample was incubated with primary antibody (1/100 in 1X PBS + 0.3% Triton X-100 + 2 % normal goat serum) for 48 hours at 4°C. ab150174, a goat anti-chicken IgY Alexa Fluor® 555 (1/500) was used as the secondary antibody (assigned red color).

    See Abreview

Anti-L Glutamate antibody (ab62668)参考文献

ab62668 has not yet been referenced specifically in any publications.

Product Wall

Application IHC - Wholemount
Sample Mouse Tissue (Whole brain)
Specification Whole brain
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Abcam user community

Verified customer

提交于 Feb 17 2014

Thanks for your call today and for your patience while I have looked into your question. I found that the antibody ab8893 does not react with free L-glutamate so I don't believe it will be suitable for the ELISA assay that you are planning. We d...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"