概述

  • 产品名称
    L-Amino Acid Assay试剂盒
  • 样品类型
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • 检测类型
    Quantitative
  • 灵敏度
    <= 40 µM
  • 检测时间
    0h 30m
  • 产品概述

    Abcam's L-Amino Acid Assay Kit provides a convenient means for directly detecting L-amino acid in biological samples. There is no requirement for sample pretreatment or purification when using this kit. The L-amino acid(s) level can be quantified using fluorometric (at Ex/Em = 535/587 nm) or colorimetric (at ? = 570 nm) methods in 96-well plates.
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    L-Amino acids are the most essential elements in biology. Accurately quantitating L-amino acids in body fluids or purified samples may provide valuable information for diagnostic or basic research studies.

  • 经测试应用
    适用于: Functional Studiesmore details

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 标识符 100 tests
    L-Amino Acid Assay Buffer WM 1 x 25ml
    L-Amino Acid Enzyme Mix Green 1 vial
    L-amino Acid Probe Red 1 x 0.2ml
    L-Amino Acid Standard (4 nmol/µl) Yellow 1 x 300µl
  • 研究领域
  • 相关性
    Amino acids perform various important functions for the body and serve as the materials for the body's cells, hormones, and enzymes. Of the standard amino acids, all by glycine can exist in either of the two optical isomers, called D and L enantiomers, which are mirror images of each other. The L-amino acids represent the vast majority of amino acids found in proteins during translation in the ribosome, and are therefore the most essential element in biology and metabolic functions.

应用

Our Abpromise guarantee covers the use of ab65347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

图片

  • Functional studies - L-Amino Acid Assay Kit (ab65347)
    Functional studies - L-Amino Acid Assay Kit (ab65347)

    Standard curve (fluorimetrical): mean of duplicates (+/-) with background subtracted

  • Functional studies - L-Amino Acid Assay Kit (ab65347)
    Functional studies - L-Amino Acid Assay Kit (ab65347)

    Standard curve (colorimetrical): mean of duplicates (+/-SD) with background readings subtracted

  • Functional Studies - L-Amino Acid Assay Kit (ab65347)
    Functional Studies - L-Amino Acid Assay Kit (ab65347)
    L-Amino Acid Standard Curve.

实验方案

文献

This product has been referenced in:

See all 2 Publications for this product

发表研究结果有使用 ab65347？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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6 reviews or Q&A

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Inquiry: Can you please assit with more detailed sample prep instructions for assay kits: - L-Amino Acid Assay Kit ab65347 - Branch Chain Amino Acid Kit ab83374 The protocol booklet does not specify (with regards to cell lization/seeding in microtitre ...

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ab83374 (Branched Chain Amino Acid Assay Kit). We have not tested this kit with cell or tissue lysates yet but only with fluids like Cell culture supernatant, Urine, Serum, Plasma and can therefore not provide more than a general protocol below. Read More

Hello,

Do you have any informations for testing muscle homogenate samples with your kits ab65347 and ab83374 ?
Thanks in advance for your help
--

Merci de nous avoir contactés.

Le laboratoire m'a confirmé que nous n'avons malheureusement pas de données expérimentales pour ces kits sur des lysats musculaires.

Désolé de ne pas pouvoir vou...

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I am wondering if you could tell me a little about the detection chemistry used in the ab65347 L-Amino Acid Assay Kit.


I am afraid that this is proprietary information which cannot be disclosed.

We apologize for any inconvenience this may cause.

Do you have any recommendations on the volume of serum I should use in this assay? I have mouse serum samples.

I general, werecommend using several doses of your serum sample to ensure the readings are within the standard curve range. In essence, the dose range selection depends on your expectation of the L-amino-acid concentration in your samples.

An...

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Please confirm which reagents are and are not compatible with kit ab65347

Thank you for your enquiry.

I am sorry to confirm we do not currently have specific data in terms of what reagents and at what concentrations would interfere with this assay. Theoretically chemicals at high concentrations and ionic detergent...

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Will this kit only detect free amino acids?

Thank you for your phone call today. I have gotten in touch with the production laboratory, and I was told that the kit only detects free L-amino acids. I hope this is helpful but please let me know if you have further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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