L-Amino Acid Assay试剂盒(ab65347)
- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
- 产品名称L-Amino Acid Assay试剂盒
- 样品类型Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
- 检测类型Quantitative
- 灵敏度<= 40 µM
- 检测时间0h 30m
- 产品概述
Abcam's L-Amino Acid Assay Kit provides a convenient means for directly detecting L-amino acid in biological samples. There is no requirement for sample pretreatment or purification when using this kit. The L-amino acid(s) level can be quantified using fluorometric (at Ex/Em = 535/587 nm) or colorimetric (at ? = 570 nm) methods in 96-well plates.
Visit our FAQs page for tips and troubleshooting. - 说明
L-Amino acids are the most essential elements in biology. Accurately quantitating L-amino acids in body fluids or purified samples may provide valuable information for diagnostic or basic research studies.
- 经测试应用适用于: Functional Studiesmore details
性能
- 存放说明Store at -20°C. Please refer to protocols.
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组件 标识符 100 tests L-Amino Acid Assay Buffer WM 1 x 25ml L-Amino Acid Enzyme Mix Green 1 vial L-amino Acid Probe Red 1 x 0.2ml L-Amino Acid Standard (4 nmol/µl) Yellow 1 x 300µl - 研究领域
- 相关性Amino acids perform various important functions for the body and serve as the materials for the body's cells, hormones, and enzymes. Of the standard amino acids, all by glycine can exist in either of the two optical isomers, called D and L enantiomers, which are mirror images of each other. The L-amino acids represent the vast majority of amino acids found in proteins during translation in the ribosome, and are therefore the most essential element in biology and metabolic functions.
应用
Our Abpromise guarantee covers the use of ab65347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies | Use at an assay dependent dilution. |
L-Amino Acid Assay Kit 图像
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Functional studies - L-Amino Acid Assay Kit (ab65347)Standard curve (fluorimetrical): mean of duplicates (+/-) with background subtracted
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Functional studies - L-Amino Acid Assay Kit (ab65347)Standard curve (colorimetrical): mean of duplicates (+/-SD) with background readings subtracted
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Functional Studies - L-Amino Acid Assay Kit (ab65347)L-Amino Acid Standard Curve.
实验方案
L-Amino Acid Assay Kit (ab65347)参考文献
This product has been referenced in:
- Zhang Y et al. Coordinated regulation of protein synthesis and degradation by mTORC1. Nature N/A:N/A (2014). Read more (PubMed: 25043031) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"