概述

性能

应用

Our Abpromise guarantee covers the use of ab90809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50. Predicted molecular weight: 143 kDa.
IP Use at an assay dependent concentration.
IHC-Fr 1/50.
ELISA Use at an assay dependent concentration.

靶标

  • 功能Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton.
  • 疾病相关Note=A chromosomal aberration involving MLLT4 is associated with acute leukemias. Translocation t(6;11)(q27;q23) with MLL/HRX. The result is a rogue activator protein.
  • 序列相似性Contains 1 dilute domain.
    Contains 1 FHA domain.
    Contains 1 PDZ (DHR) domain.
    Contains 2 Ras-associating domains.
  • 结构域The PDZ/DHR domain interacts with the C-terminus of nectin and the Pro-rich N-terminus domain interacts with F-actin.
  • 细胞定位Cell junction > adherens junction. Not found at cell-matrix AJs.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AF6 antibody
    • AFAD_HUMAN antibody
    • Afadin antibody
    • ALL1-fused gene from chromosome 6 protein antibody
    • Mllt4 antibody
    • Protein Af-6 antibody
    • Protein AF6 antibody
    see all

Anti-l Afadin antibody [3] (ab90809)参考文献

This product has been referenced in:
  • Poon CE  et al. Nectin-3 Is Increased in the Cell Junctions of the Uterine Epithelium at Implantation. Reprod Sci 23:1580-1592 (2016). Read more (PubMed: 27217376) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MDA-MB-231 cells)
Loading amount 100 µg
Specification MDA-MB-231 cells
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 8 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 6-8°C
Username

Abcam user community

Verified customer

提交于 May 11 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"