Anti-kynurenine 3-monooxygenase抗体(ab83929)

概述

  • 产品名称Anti-kynurenine 3-monooxygenase抗体
    参阅全部 kynurenine 3-monooxygenase 一抗
  • 描述
    兔多克隆抗体to kynurenine 3-monooxygenase
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Synthetic peptide, within internal sequence amino acids 200-299 of Mouse kynurenine 3-monooxygenase

  • 阳性对照
    • Mouse brain extract.

性能

应用

Our Abpromise guarantee covers the use of ab83929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
ELISA Use at an assay dependent dilution.

靶标

  • 功能Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid, a neurotoxic NMDA receptor antagonist and potential endogenous inhibitor of NMDA receptor signaling in axonal targeting, synaptogenesis and apoptosis during brain development. Quinolinic acid may also affect NMDA receptor signaling in pancreatic beta cells, osteoblasts, myocardial cells, and the gastrointestinal tract.
  • 组织特异性Highest levels in placenta and liver. Detectable in kidney.
  • 通路Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 1/3.
  • 序列相似性Belongs to the aromatic-ring hydroxylase family. KMO subfamily.
  • 细胞定位Mitochondrion outer membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • dJ317G22.1 antibody
    • kmo antibody
    • KMO_HUMAN antibody
    • Kynurenine 3 hydroxylase antibody
    • Kynurenine 3 monooxygenase (kynurenine 3 hydroxylase) antibody
    • Kynurenine 3 monooxygenase antibody
    • Kynurenine 3-hydroxylase antibody
    • Kynurenine 3-monooxygenase antibody
    • RP1-317G22.1 antibody
    see all

Anti-kynurenine 3-monooxygenase antibody 图像

  • Anti-kynurenine 3-monooxygenase antibody (ab83929) at 1 µg/ml + Mouse brain extract

    Predicted band size : 56 kDa
    Observed band size : 56 kDa

Anti-kynurenine 3-monooxygenase antibody (ab83929)参考文献

ab83929 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Purified protein (Brain, Hyp and Hpc)
Loading amount 30 µg
Specification Brain, Hyp and Hpc
Treatment Prenatal stress paradigm
Gel Running Conditions Reduced Denaturing (7.5% TrisHCl)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Apr 23 2013

Thank you for the update. Our datasheet shows a nice clean blot of mouse brain, so it is frustrating that you do not get that result with your brain samples. The placenta result may be real but given the other bands in that blot, I would be skeptical. ...

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Thank you for your enquiry.

I have obtained some further information regarding the WB image on the datasheet.

The sample were extracts of mouse brain, each lane represents approximately 20 ug of extract. The mouse brain extract wa...

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