Anti-kynurenine 3-monooxygenase抗体(ab83929)

概述

  • 产品名称
    Anti-kynurenine 3-monooxygenase抗体
    参阅全部 kynurenine 3-monooxygenase 一抗
  • 描述
    兔多克隆抗体to kynurenine 3-monooxygenase
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Synthetic peptide, within internal sequence amino acids 200-299 of Mouse kynurenine 3-monooxygenase

  • 阳性对照
    • Mouse brain extract.

性能

应用

Our Abpromise guarantee covers the use of ab83929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
ELISA Use at an assay dependent dilution.

靶标

  • 功能
    Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid, a neurotoxic NMDA receptor antagonist and potential endogenous inhibitor of NMDA receptor signaling in axonal targeting, synaptogenesis and apoptosis during brain development. Quinolinic acid may also affect NMDA receptor signaling in pancreatic beta cells, osteoblasts, myocardial cells, and the gastrointestinal tract.
  • 组织特异性
    Highest levels in placenta and liver. Detectable in kidney.
  • 通路
    Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 1/3.
  • 序列相似性
    Belongs to the aromatic-ring hydroxylase family. KMO subfamily.
  • 细胞定位
    Mitochondrion outer membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • dJ317G22.1 antibody
    • kmo antibody
    • KMO_HUMAN antibody
    • Kynurenine 3 hydroxylase antibody
    • Kynurenine 3 monooxygenase (kynurenine 3 hydroxylase) antibody
    • Kynurenine 3 monooxygenase antibody
    • Kynurenine 3-hydroxylase antibody
    • Kynurenine 3-monooxygenase antibody
    • RP1-317G22.1 antibody
    see all

Anti-kynurenine 3-monooxygenase antibody 图像

  • Anti-kynurenine 3-monooxygenase antibody (ab83929) at 1 µg/ml + Mouse brain extract

    Predicted band size : 56 kDa
    Observed band size : 56 kDa

实验方案

Anti-kynurenine 3-monooxygenase antibody (ab83929)参考文献

ab83929 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Purified protein (Brain, Hyp and Hpc)
Loading amount
30 µg
Specification
Brain, Hyp and Hpc
Treatment
Prenatal stress paradigm
Gel Running Conditions
Reduced Denaturing (7.5% TrisHCl)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Apr 23 2013

Thank you for the update. Our datasheet shows a nice clean blot of mouse brain, so it is frustrating that you do not get that result with your brain samples. The placenta result may be real but given the other bands in that blot, I would be skeptical. ...

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Thank you for your enquiry.

I have obtained some further information regarding the WB image on the datasheet.

The sample were extracts of mouse brain, each lane represents approximately 20 ug of extract. The mouse brain extract wa...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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