概述
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产品名称Anti-Kv2.1 (phospho S567)抗体
参阅全部 Kv2.1 一抗 -
描述兔多克隆抗体to Kv2.1 (phospho S567)
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宿主Rabbit
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特异性ab61107 detects endogenous levels of Kv2.1/KCNB1 only when phosphorylated at Serine 567.
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经测试应用适用于: ELISA, IHC-Pmore details
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种属反应性与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human Kv2.1.
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阳性对照
- Human brain tissue.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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纯化说明The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab61107 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA | 1/5000. | |
| IHC-P | 1/50 - 1/100. |
靶标
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功能Mediates the voltage-dependent potassium ion permeability of excitable membranes. Channels open or close in response to the voltage difference across the membrane, letting potassium ions pass in accordance with their electrochemical gradient.
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序列相似性Belongs to the potassium channel family. B (Shab) (TC 1.A.1.2) subfamily. Kv2.1/KCNB1 sub-subfamily.
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结构域The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
The tail may be important in modulation of channel activity and/or targeting of the channel to specific subcellular compartments. -
翻译后修饰Highly phosphorylated on serine residues in the C-terminal. Differential phosphorylation on a subset of serines allows graded activity-dependent regulation of channel gating. Phosphorylation on Ser-457, Ser-541, Ser-567, Ser-607, Ser-656 and Ser-720 as well as the N-terminal Ser-15 are all regulated by calcineurin-mediated dephosphorylation. Particularly, Ser-607 and Tyr-128 are significant sites of voltage-gated regulation through phosphorylation/ dephosphorylation activities. Tyr-128 can be dephosphorylated by PTPalpha and cyt-PTPepsilon. Phosphorylation levels on Ser-607 are supersensitive to neuronal activity. Phosphorylation on Ser-567 is reduced during postnatal development with low levels at P2 and P5. Levels then increase to reach adult levels by P14. Phosphorylation levels on Ser-564 and Ser-607 are greatly reduced during seizures, by 40% and 85% respectively.
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细胞定位Membrane.
- Information by UniProt
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数据库链接
- Entrez Gene: 3745 Human
- Entrez Gene: 16500 Mouse
- Entrez Gene: 25736 Rat
- Omim: 600397 Human
- SwissProt: Q14721 Human
- SwissProt: Q03717 Mouse
- SwissProt: P15387 Rat
- Unigene: 84244 Human
see all -
别名
- Delayed rectifier potassium channel 1 antibody
- Delayed rectifier potassium channel Kv2.1 antibody
- DRK 1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv2.1 (phospho S567) antibody (ab61107)ab61107, at a 1/50-1/100 dilution, staining human KCNB1 in brain tissue with(+) or without(-) immunising phospopeptide, using Immunohistochemistry, Paraffin embedded tissue
文献
ab61107 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"