概述

  • 产品名称Anti-Kv2.1抗体
    参阅全部 Kv2.1 一抗
  • 描述
    兔多克隆抗体to Kv2.1
  • 特异性ab111122 detects endogenous levels of total Kv2.1 protein.
  • 经测试应用适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from an internal sequence of Human Kv2.1.

  • 阳性对照
    • COS7 cells; Human brain tissue

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.88% Sodium chloride, 49.1% PBS
    Note: PBS is without Mg2+ and Ca2+
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab111122 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab111122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

靶标

  • 功能Mediates the voltage-dependent potassium ion permeability of excitable membranes. Channels open or close in response to the voltage difference across the membrane, letting potassium ions pass in accordance with their electrochemical gradient.
  • 序列相似性Belongs to the potassium channel family. B (Shab) (TC 1.A.1.2) subfamily. Kv2.1/KCNB1 sub-subfamily.
  • 结构域The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
    The tail may be important in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
  • 翻译后修饰Highly phosphorylated on serine residues in the C-terminal. Differential phosphorylation on a subset of serines allows graded activity-dependent regulation of channel gating. Phosphorylation on Ser-457, Ser-541, Ser-567, Ser-607, Ser-656 and Ser-720 as well as the N-terminal Ser-15 are all regulated by calcineurin-mediated dephosphorylation. Particularly, Ser-607 and Tyr-128 are significant sites of voltage-gated regulation through phosphorylation/ dephosphorylation activities. Tyr-128 can be dephosphorylated by PTPalpha and cyt-PTPepsilon. Phosphorylation levels on Ser-607 are supersensitive to neuronal activity. Phosphorylation on Ser-567 is reduced during postnatal development with low levels at P2 and P5. Levels then increase to reach adult levels by P14. Phosphorylation levels on Ser-564 and Ser-607 are greatly reduced during seizures, by 40% and 85% respectively.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Delayed rectifier potassium channel 1 antibody
    • Delayed rectifier potassium channel Kv2.1 antibody
    • DRK 1 antibody
    • DRK1 antibody
    • h DRK1 K(+) channel antibody
    • h-DRK1 antibody
    • hDRK 1 antibody
    • hDRK1 antibody
    • KCB 1 antibody
    • KCB1 antibody
    • KCNB1 antibody
    • KCNB1_HUMAN antibody
    • KV2.1 antibody
    • Potassium channel protein DRK1 antibody
    • Potassium voltage gated channel shab related subfamily member 1 antibody
    • Potassium voltage-gated channel subfamily B member 1 antibody
    • Voltage-gated potassium channel subunit Kv2.1 antibody
    see all

Anti-Kv2.1 antibody 图像

  • Immunofluorescence analysis of Kv2.1 in COS7 cells, using ab111122 at a 1/100 dilution. The image on the right is from cells treated with the synthesized peptide.
  • ab111122 at 1/50 dilution staining Kv2.1 in paraffin embedded Human brain tissue by Immunohistochemistry. The image on the right is from cells treated with the synthesized peptide.

Anti-Kv2.1 antibody (ab111122)参考文献

This product has been referenced in:
  • Kim SH  et al. Electrogenic transport and K(+) ion channel expression by the human endolymphatic sac epithelium. Sci Rep 5:18110 (2015). IHC ; Human . Read more (PubMed: 26655723) »

See 1 Publication for this product

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