Anti-Kv1.6抗体(ab21089)

概述

  • 产品名称Anti-Kv1.6抗体
    参阅全部 Kv1.6 一抗
  • 描述
    兔多克隆抗体to Kv1.6
  • 特异性In human HEK293 cells ab21089 recognised a band of ~58 kDa, the predicted MW of Kv1.6 (data not shown). This band was not blocked by the immunising peptide. Additional bands of 100 kDa, 25 kDa and 20 kDa were also recognised.
  • 经测试应用适用于: ICC/IF, WB, IHC-FoFr, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human Kv1.6.

    (Peptide available as ab23431.)

  • 阳性对照
    • This antibody gave a positive signal in Hek293 cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab21089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
IHC-FoFr 1/3000.
IHC-P 1/40. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

靶标

  • 功能Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.
  • 序列相似性Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.6/KCNA6 sub-subfamily.
  • 结构域The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HBK 2 antibody
    • HBK2 antibody
    • Human brain potassium channel 2 antibody
    • KCNA 6 antibody
    • Kcna6 antibody
    • KCNA6_HUMAN antibody
    • Potassium voltage gated channel shaker related subfamily member 6 antibody
    • Potassium voltage gated channel subfamily A member 6 antibody
    • Potassium voltage-gated channel subfamily A member 6 antibody
    • Voltage gated potassium channel protein Kv1.6 antibody
    • Voltage gated potassium channel subunit Kv1.6 antibody
    • Voltage-gated potassium channel HBK2 antibody
    • Voltage-gated potassium channel subunit Kv1.6 antibody
    see all

Anti-Kv1.6 antibody 图像

  • ICC/IF image of ab21089 stained human HEK 293 cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab21089, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • Image courtesy of Human Protein Atlas

    ab21089 staining Kv6.1 in human pancreas tissue. Paraffin embedded human pancreas tissue was incubated with ab21089 (1/40 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab21089 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

    .

  • The IHC-FoFr image stainig Kv1.6(ab21089) on Rat DRG sections. The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the tissues were cryoprotected in sucrose 30% overnight. Tissues were then cut using a cryostat.

    See Abreview

Anti-Kv1.6 antibody (ab21089)参考文献

ab21089 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (DRG)
Specification DRG
Fixative Paraformaldehyde
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Dr. Sophie Pezet

Verified customer

提交于 Sep 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"