Anti-Kv1.6/RCK2抗体(ab21089)
Key features and details
- Rabbit polyclonal to Kv1.6/RCK2
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Kv1.6/RCK2抗体 -
描述
兔多克隆抗体to Kv1.6/RCK2 -
宿主
Rabbit -
特异性
In human HEK293 cells ab21089 recognised a band of ~58 kDa, the predicted MW of Kv1.6/RCK2 (data not shown). This band was not blocked by the immunising peptide. Additional bands of 100 kDa, 25 kDa and 20 kDa were also recognised.
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经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
Synthetic peptide corresponding to Human Kv1.6/RCK2 aa 500 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab23431) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab21089于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 5 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
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说明 |
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ICC/IF
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). |
靶标
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功能
Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient. -
序列相似性
Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.6/KCNA6 sub-subfamily. -
结构域
The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3742 Human
- Entrez Gene: 16494 Mouse
- Entrez Gene: 64358 Rat
- Omim: 176257 Human
- SwissProt: P17658 Human
- SwissProt: Q61923 Mouse
- SwissProt: P17659 Rat
- Unigene: 306190 Human
see all -
别名
- HBK 2 antibody
- HBK2 antibody
- Human brain potassium channel 2 antibody
see all
图片
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All lanes : Anti-Kv1.6/RCK2 antibody (ab21089) at 1 µg/ml
Lane 1 : Mouse Brain Lysate
Lane 2 : Mouse Brain Lysate Kv1.6/RCK2 with Human Kv1.6/RCK2 peptide (ab23431) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Lane 1 : Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Lane 2 : Alexa Fluor Goat polyclonal to Rabbit IgG
Predicted band size: 58 kDaab21089 recognises a band corresponding to the predicted MW of Kv1.6/RCK2 in mouse brain lysate (lane 1). Antibody binding to this band may be blocked using the immunising peptide, ab23431 (lane 2). The antibody also recognises additional proteins. These may be degradation/cleavage products of Kv1.6/RCK2, or they may represent non-specific binding by the antibody.
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ICC/IF image of ab21089 stained human HEK (Human embryonic kidney) 293 cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab21089, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab21089 被引用在 1 文献中.
- Suda M et al. Adult-onset hypothyroidism causes mechanical hypersensitivity due to peripheral nerve hyperexcitability based on voltage-gated potassium channel downregulation in male mice. J Neurosci Res 100:506-521 (2022). PubMed: 34935172