Anti-Kv1.4抗体(ab16718)

概述

  • 产品名称Anti-Kv1.4抗体
    参阅全部 Kv1.4 一抗
  • 描述
    兔多克隆抗体to Kv1.4
  • 经测试应用适用于: ELISA, IP, ICC/IF, WBmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Cow
  • 免疫原

    Recombinant fragment:

    CNSHMPYGYAAQARARERERLAHSR

    , corresponding to amino acids 13-37 of Rat Kv1.4 (conjugated to KLH).

  • 阳性对照
    • Rat hippocampal membranes and lysates of HEK293 cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.08% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度IgG fraction
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab16718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
IP Use a concentration of 1 - 10 µg/ml.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 73 kDa. Detects a band of approximately 76 KDa in HEK293 lysate and 90 kDa in rat hippocampal membranes.

靶标

  • 功能Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.
  • 序列相似性Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.4/KCNA4 sub-subfamily.
  • 结构域The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Voltage gated K+ channel HuKII antibody
    • Fetal skeletal muscle potassium channel antibody
    • HBK4 antibody
    • HK1 antibody
    • HPCN2 antibody
    • HUKII antibody
    • Kcna4 antibody
    • KCNA4_HUMAN antibody
    • KCNA4L antibody
    • KCNA8 antibody
    • kv1.4 antibody
    • PCN2 antibody
    • Potassium channel 2 antibody
    • Potassium voltage gated channel shaker related subfamily member 4 antibody
    • Potassium voltage gated channel subfamily A member 4 antibody
    • Potassium voltage-gated channel subfamily A member 4 antibody
    • Rapidly inactivating potassium channel antibody
    • Shaker related potassium channel Kv1.4 antibody
    • Type A potassium channel antibody
    • Voltage gated potassium channel HBK4 antibody
    • Voltage gated potassium channel HK1 antibody
    • Voltage gated potassium channel subunit Kv1.4 antibody
    • Voltage-gated K(+) channel HuKII antibody
    • Voltage-gated potassium channel HBK4 antibody
    • Voltage-gated potassium channel HK1 antibody
    • Voltage-gated potassium channel subunit Kv1.4 antibody
    see all

Anti-Kv1.4 antibody 图像

  • Western blots using ab16718.

    1: HEK293 cells transfected with Kv1.4.

    2: Rat hippocampal membrane.

    The size difference observed can probably be explained by differential post-translational processing.

  • ab16718 detecting rat recombinant protein by ELISA. Kv1.4 amino terminal domain was immobilized on 96-well plate using streptavidin-biotin interaction. Wells were blocked in 1% milk for 30 minutes at 19°C. The primary antibody was diluted 1/500 & 1/750 in PBS-Tween(0.1%) and incubated for 1 hour at 19°C. HRP conjugated goat monoclonal to rabbit, 1/2000 dilution was used as secondary.

    See Abreview

Anti-Kv1.4 antibody (ab16718)参考文献

ab16718 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Rat Recombinant protein (E.Coli)
Specification E.Coli
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 19°C
Type Indirect
Username

Abcam user community

Verified customer

提交于 Nov 18 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Recombinant protein (E.Coli)
Loading amount 0.1 µg
Specification E.Coli
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Aug 27 2008

Thank you for your enquiry. I apologize for the delay in finding out this information, I can now inform you that this antibody was raised against a peptide which was conjugated to KLH for immunization. I hope this information will be useful, please...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"