Anti-Kv1.2抗体- Carboxyterminal end (ab55987)

概述

  • 产品名称Anti-Kv1.2抗体- Carboxyterminal end
    参阅全部 Kv1.2 一抗
  • 描述
    兔多克隆抗体to Kv1.2 - Carboxyterminal end
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Recombinant Fragment
    预测可用于: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to C-terminal residues of mouse KCNA2

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab55987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 57 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent dilution.
ICC/IF Use a concentration of 1 - 5 µg/ml.

靶标

  • 相关性Kv1.2 mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which K(+) ions may pass in accordance with their electrochemical gradient. Kv1.2 binds PDZ domains of DLG1, DLG2 and DLG4. The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
  • 细胞定位Membrane; Multi-pass membrane protein
  • 数据库链接
  • 别名
    • Akr6a4 antibody
    • BK2 antibody
    • HBK5 antibody
    • HK4 antibody
    • HUKIV antibody
    • Kca1 2 antibody
    • kcna2 antibody
    • KV1.2 antibody
    • MGC50217 antibody
    • Mk 2 antibody
    • MK2 antibody
    • NGK1 antibody
    • Potassium voltage gated channel shaker related subfamily member 2 antibody
    • Potassium voltage-gated channel subfamily A member 2 antibody
    • RBK2 antibody
    • Voltage gated potassium channel subunit Kv1.2 antibody
    see all

Anti-Kv1.2 antibody - Carboxyterminal end 图像

  • ICC/IF image of ab55987 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55987, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Kv1.2 antibody - Carboxyterminal end (ab55987)参考文献

ab55987 has not yet been referenced specifically in any publications.

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