Anti-Ku80 (phospho T714)抗体(ab55350)


  • 产品名称Anti-Ku80 (phospho T714)抗体
    参阅全部 Ku80 一抗
  • 描述
    兔多克隆抗体to Ku80 (phospho T714)
  • 特异性This antibody detects endogenous levels of Ku80 only when phosphorylated at Threonine 714.
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human Ku80 around the phosphorylation site of Threonine 714 (GDTPAA)

  • 阳性对照
    • COS7 cells


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明 The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab55350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000.
WB 1/500 - 1/1000. Predicted molecular weight: 80 kDa.


  • 功能Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
  • 序列相似性Belongs to the ku80 family.
    Contains 1 Ku domain.
  • 发展阶段Expression increases during promyelocyte differentiation.
  • 结构域The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.
  • 翻译后修饰Phosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity.
  • 细胞定位Nucleus. Chromosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 86 kDa subunit of Ku antigen antibody
    • ATP dependent DNA helicase 2 subunit 2 antibody
    • ATP dependent DNA helicase II 80 kDa subunit antibody
    • ATP dependent DNA helicase II 86 Kd subunit antibody
    • ATP dependent DNA helicase II antibody
    • ATP-dependent DNA helicase 2 subunit 2 antibody
    • ATP-dependent DNA helicase II 80 kDa subunit antibody
    • CTC box binding factor 85 kDa antibody
    • CTC box-binding factor 85 kDa subunit antibody
    • CTC85 antibody
    • CTCBF antibody
    • DNA repair protein XRCC5 antibody
    • KARP 1 antibody
    • KARP1 antibody
    • Ku 80 antibody
    • Ku autoantigen 80kDa antibody
    • Ku80 antibody
    • Ku86 antibody
    • Ku86 autoantigen related protein 1 antibody
    • KUB 2 antibody
    • KUB2 antibody
    • Lupus Ku autoantigen protein p86 antibody
    • NFIV antibody
    • Nuclear factor IV antibody
    • Thyroid lupus autoantigen antibody
    • Thyroid-lupus autoantigen antibody
    • TLAA antibody
    • X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining) antibody
    • X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) antibody
    • X-ray repair cross-complementing protein 5 antibody
    • Xray repair complementing defective repair in Chinese hamster cells 5 antibody
    • XRCC 5 antibody
    • XRCC5 antibody
    • XRCC5_HUMAN antibody
    see all

Anti-Ku80 (phospho T714) antibody 图像

  • All lanes : Anti-Ku80 (phospho T714) antibody (ab55350) at 1/500 dilution

    Lane 1 : extracts from COS7 cells with no immunizing peptide
    Lane 2 : extracts from COS7 cells with immunizing peptide

    Predicted band size : 80 kDa

Anti-Ku80 (phospho T714) antibody (ab55350)参考文献

ab55350 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human fibroblasts)
Specification Human fibroblasts
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Dr. David Maranon

Verified customer

提交于 Jan 27 2011