Anti-Ku80抗体[EPR3468] (ab80592)

概述

• 产品名称Anti-Ku80抗体[EPR3468]
  参阅全部 Ku80 一抗
• 描述
  兔单克隆抗体[EPR3468] to Ku80
• 经测试应用适用于: WB, IP, IHC-P, ICC/IF, Flow Cytmore details
• 种属反应性
  与反应: Human
  
• 免疫原

  Synthetic peptide (Human)

• 阳性对照
  • WB: lysates from A549, HeLa, HepG2 and MCF7 cells; IHC-P: Human tonsil tissue; ICC/IF: HeLa cells. FC: HeLa cells.
• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

   

  Alternative versions available:

  Anti-Ku80 antibody (Alexa Fluor^® 488) [EPR3468] (ab198586)
  Anti-Ku80 antibody (Alexa Fluor^® 647) [EPR3468] (ab198587)

  Anti-Ku80 antibody (HRP) [EPR3468] (ab199096)

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
• 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• 纯度Tissue culture supernatant
• 克隆单克隆
• 克隆编号EPR3468
• 同种型IgG
• 研究领域
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • Non Homol. End Joining

相关产品

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
• Positive Controls
  • HeLa nuclear extract lysate (ab14655)
  • HepG2 nuclear extract lysate (ab14660)
  • MCF7 nuclear extract lysate (ab14860)

应用

靶标

• 功能Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
• 序列相似性Belongs to the ku80 family.
  Contains 1 Ku domain.
• 发展阶段Expression increases during promyelocyte differentiation.
• 结构域The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.
• 翻译后修饰Phosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity.
  Sumoylated.
• 细胞定位Nucleus. Chromosome.

• Target information above from: UniProt accession P13010 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 数据库链接
  • Entrez Gene: 7520 Human
  • Omim: 194364 Human
  • SwissProt: P13010 Human
  • Unigene: 388739 Human
• 别名
  • 86 kDa subunit of Ku antigen antibody
  • ATP dependent DNA helicase 2 subunit 2 antibody
  • ATP dependent DNA helicase II 80 kDa subunit antibody

  • ATP dependent DNA helicase II 86 Kd subunit antibody
  • ATP dependent DNA helicase II antibody
  • ATP-dependent DNA helicase 2 subunit 2 antibody
  • ATP-dependent DNA helicase II 80 kDa subunit antibody
  • CTC box binding factor 85 kDa antibody
  • CTC box-binding factor 85 kDa subunit antibody
  • CTC85 antibody
  • CTCBF antibody
  • DNA repair protein XRCC5 antibody
  • KARP 1 antibody
  • KARP1 antibody
  • Ku 80 antibody
  • Ku autoantigen 80kDa antibody
  • Ku80 antibody
  • Ku86 antibody
  • Ku86 autoantigen related protein 1 antibody
  • KUB 2 antibody
  • KUB2 antibody
  • Lupus Ku autoantigen protein p86 antibody
  • NFIV antibody
  • Nuclear factor IV antibody
  • Thyroid lupus autoantigen antibody
  • Thyroid-lupus autoantigen antibody
  • TLAA antibody
  • X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining) antibody
  • X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) antibody
  • X-ray repair cross-complementing protein 5 antibody
  • Xray repair complementing defective repair in Chinese hamster cells 5 antibody
  • XRCC 5 antibody
  • XRCC5 antibody
  • XRCC5_HUMAN antibody

  see all