- 数据表
- 具体的参考文献 (4)
- 实验方案
概述
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产品名称Anti-Ku80抗体[EPR3468]
参阅全部 Ku80 一抗 -
描述兔单克隆抗体[EPR3468] to Ku80
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经测试应用适用于: WB, IP, IHC-P, ICC/IF, Flow Cytmore details
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种属反应性与反应: Human
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免疫原
Synthetic peptide within Human Ku80 aa 700-800. The exact sequence is proprietary.
Database link: P13010 -
阳性对照
- WB: lysates from A549, HeLa, HepG2 and MCF7 cells; IHC-P: Human tonsil tissue; ICC/IF: HeLa cells. FC: HeLa cells.
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常规说明
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
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存储溶液pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 50% Glycerol, 0.05% BSA -
纯度Tissue culture supernatant
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克隆单克隆
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克隆编号EPR3468
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同种型IgG
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研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab80592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 83 kDa. | |
| IP | 1/50. | |
| IHC-P | 1/250 - 1/500. | |
| ICC/IF | 1/500 - 1/1000. | |
| Flow Cyt | 1/20. |
靶标
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功能Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
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序列相似性Belongs to the ku80 family.
Contains 1 Ku domain. -
发展阶段Expression increases during promyelocyte differentiation.
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结构域The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.
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翻译后修饰Phosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity.
Sumoylated. -
细胞定位Nucleus. Chromosome.
- Information by UniProt
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数据库链接
- Entrez Gene: 7520 Human
- Omim: 194364 Human
- SwissProt: P13010 Human
- Unigene: 388739 Human
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别名
- 86 kDa subunit of Ku antigen antibody
- ATP dependent DNA helicase 2 subunit 2 antibody
- ATP dependent DNA helicase II 80 kDa subunit antibody
see all
Anti-Ku80 antibody [EPR3468] 图像
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![Western blot - Ku80 antibody [EPR3468] (ab80592)](http://www.abcam.cn/ps/products/80/ab80592/Images/Ku80-Primary-antibodies-ab80592-1.jpg)
Western blot - Ku80 antibody [EPR3468] (ab80592)All lanes : Anti-Ku80 antibody [EPR3468] (ab80592) at 1/50000 dilution
Lane 1 : A549 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 83 kDa
Observed band size : 83 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ku80 antibody [EPR3468] (ab80592)](http://www.abcam.cn/ps/products/80/ab80592/Images/Ku80-Primary-antibodies-ab80592-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ku80 antibody [EPR3468] (ab80592)Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using 1/100 ab80592. -
![Immunocytochemistry/ Immunofluorescence - Ku80 antibody [EPR3468] (ab80592)](http://www.abcam.cn/ps/products/80/ab80592/Images/Ku80-Primary-antibodies-ab80592-3.jpg)
Immunocytochemistry/ Immunofluorescence - Ku80 antibody [EPR3468] (ab80592)Immunofluorescence analysis of HeLa cells with 1/500 ab80592. -
![Flow Cytometry - Anti-Ku80 antibody [EPR3468] (ab80592)](http://www.abcam.cn/ps/products/80/ab80592/Images/ab80592-271362-ab80592FC1.jpg)
Flow Cytometry - Anti-Ku80 antibody [EPR3468] (ab80592)Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Ku80 with unpurified ab80592 at 1/20 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
实验方案
Anti-Ku80 antibody [EPR3468] (ab80592)参考文献
This product has been referenced in:
- Wong RCB et al. Mitochondrial replacement in an iPSC model of Leber's hereditary optic neuropathy. Aging (Albany NY) 9:1341-1350 (2017). IHC ; Human . Read more (PubMed: 28455970) »
- Ho SR et al. RNF144A, an E3 ubiquitin ligase for DNA-PKcs, promotes apoptosis during DNA damage. Proc Natl Acad Sci U S A 111:E2646-55 (2014). Read more (PubMed: 24979766) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"