概述

  • 产品名称Anti-Ku80抗体
    参阅全部 Ku80 一抗
  • 描述
    兔多克隆抗体to Ku80
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Monkey, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Ku80.

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; HEK293; A549.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab87860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).

靶标

  • 功能Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
  • 序列相似性Belongs to the ku80 family.
    Contains 1 Ku domain.
  • 发展阶段Expression increases during promyelocyte differentiation.
  • 结构域The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.
  • 翻译后修饰Phosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity.
    Sumoylated.
  • 细胞定位Nucleus. Chromosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 86 kDa subunit of Ku antigen antibody
    • ATP dependent DNA helicase 2 subunit 2 antibody
    • ATP dependent DNA helicase II 80 kDa subunit antibody
    • ATP dependent DNA helicase II 86 Kd subunit antibody
    • ATP dependent DNA helicase II antibody
    • ATP-dependent DNA helicase 2 subunit 2 antibody
    • ATP-dependent DNA helicase II 80 kDa subunit antibody
    • CTC box binding factor 85 kDa antibody
    • CTC box-binding factor 85 kDa subunit antibody
    • CTC85 antibody
    • CTCBF antibody
    • DNA repair protein XRCC5 antibody
    • KARP 1 antibody
    • KARP1 antibody
    • Ku 80 antibody
    • Ku autoantigen 80kDa antibody
    • Ku80 antibody
    • Ku86 antibody
    • Ku86 autoantigen related protein 1 antibody
    • KUB 2 antibody
    • KUB2 antibody
    • Lupus Ku autoantigen protein p86 antibody
    • NFIV antibody
    • Nuclear factor IV antibody
    • Thyroid lupus autoantigen antibody
    • Thyroid-lupus autoantigen antibody
    • TLAA antibody
    • X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining) antibody
    • X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) antibody
    • X-ray repair cross-complementing protein 5 antibody
    • Xray repair complementing defective repair in Chinese hamster cells 5 antibody
    • XRCC 5 antibody
    • XRCC5 antibody
    • XRCC5_HUMAN antibody
    see all

Anti-Ku80 antibody 图像

  • All lanes : Anti-Ku80 antibody (ab87860) at 1 µg/ml

    Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 83 kDa
    Observed band size : 83 kDa
    Additional bands at : 47 kDa,58 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

Anti-Ku80 antibody (ab87860)参考文献

ab87860 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X100 in PBS
Username

Dr. Kirk McManus

Verified customer

提交于 Apr 16 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Username

Dr. Kirk McManus

Verified customer

提交于 Mar 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"