概述

  • 产品名称Anti-Ku80抗体[111] (Biotin)
    参阅全部 Ku80 一抗
  • 描述
    小鼠单克隆抗体[111] to Ku80 (Biotin)
  • 偶联物Biotin
  • 经测试应用适用于: WB, IP, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Human, Monkey
    不与反应: Mouse, Rat, Rabbit, Cow
  • 免疫原

    Human B cell nuclei from plasmacytoid 2p68 cells

  • 表位aa 610-705
  • 阳性对照
    • HeLa, T47D or BT474 cell lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...
  • 纯度Ammonium Sulphate Precipitation
  • 克隆单克隆
  • 克隆编号111
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab79220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 83 kDa.
IP Use at 2 µg/mg of lysate.
Flow Cyt Use at an assay dependent concentration. ab18434-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
  • 序列相似性Belongs to the ku80 family.
    Contains 1 Ku domain.
  • 发展阶段Expression increases during promyelocyte differentiation.
  • 结构域The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.
  • 翻译后修饰Phosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity.
    Sumoylated.
  • 细胞定位Nucleus. Chromosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 86 kDa subunit of Ku antigen antibody
    • ATP dependent DNA helicase 2 subunit 2 antibody
    • ATP dependent DNA helicase II 80 kDa subunit antibody
    • ATP dependent DNA helicase II 86 Kd subunit antibody
    • ATP dependent DNA helicase II antibody
    • ATP-dependent DNA helicase 2 subunit 2 antibody
    • ATP-dependent DNA helicase II 80 kDa subunit antibody
    • CTC box binding factor 85 kDa antibody
    • CTC box-binding factor 85 kDa subunit antibody
    • CTC85 antibody
    • CTCBF antibody
    • DNA repair protein XRCC5 antibody
    • KARP 1 antibody
    • KARP1 antibody
    • Ku 80 antibody
    • Ku autoantigen 80kDa antibody
    • Ku80 antibody
    • Ku86 antibody
    • Ku86 autoantigen related protein 1 antibody
    • KUB 2 antibody
    • KUB2 antibody
    • Lupus Ku autoantigen protein p86 antibody
    • NFIV antibody
    • Nuclear factor IV antibody
    • Thyroid lupus autoantigen antibody
    • Thyroid-lupus autoantigen antibody
    • TLAA antibody
    • X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining) antibody
    • X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) antibody
    • X-ray repair cross-complementing protein 5 antibody
    • Xray repair complementing defective repair in Chinese hamster cells 5 antibody
    • XRCC 5 antibody
    • XRCC5 antibody
    • XRCC5_HUMAN antibody
    see all

Anti-Ku80 antibody [111] (Biotin) (ab79220)参考文献

This product has been referenced in:
  • Wang J  et al. Murine monoclonal antibodies specific for conserved and non-conserved antigenic determinants of the human and murine Ku autoantigens. Mol Biol Rep 18:15-28 (1993). Read more (PubMed: 7694076) »

See 1 Publication for this product

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