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Our Abpromise guarantee covers the use of ab3108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.5µg for 106 cells.|
|IHC-P||Use a concentration of 1 - 2 µg/ml. Antigen retrieval is not essential but may optimise staining.|
|IP||Use at 2 µg/mg of lysate.|
ab3108 at a 1/200 dilution staining asynchronous HeLa by ICC/IF. The cells were bleomycin treated for 2 hours prior to formaldehyde fixation and then labelled with antibody for 30 minutes. Bound antibody was detected using a Cy3 conjugated goat anti-mouse antibody. Nuclei were visualised using DAPI staining. Ab3108 was found to stain both treated and untreated cells very well.
This image is courtesy of an Abreview submitted by Kirk McManus.