RabMAb

Anti-Ku70抗体[EPR4026] (ab108604)

概述

  • 产品名称Anti-Ku70抗体[EPR4026]
    参阅全部 Ku70 一抗
  • 描述
    兔单克隆抗体[EPR4026] to Ku70
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide, corresponding to internal sequence amino acids 80-105 of Human Ku70

  • 阳性对照
    • HeLa, HepG2, Raji, A673, A549 and A431 cell lysates; Human breast carcinoma tissue; HeLa cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab108604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
IHC-P 1/100 - 1/250. Antigen retrieval is recommended.
ICC/IF 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
    • 序列相似性Belongs to the ku70 family.
      Contains 1 Ku domain.
      Contains 1 SAP domain.
    • 发展阶段Expression does not increase during promyelocyte differentiation.
    • 翻译后修饰Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair.
    • 细胞定位Nucleus. Chromosome.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 5''-deoxyribose-5-phosphate lyase Ku70 antibody
      • 5''-dRP lyase Ku70 antibody
      • 70 kDa subunit of Ku antigen antibody
      • ATP dependent DNA helicase 2 subunit 1 antibody
      • ATP dependent DNA helicase II 70 kDa subunit antibody
      • ATP-dependent DNA helicase 2 subunit 1 antibody
      • ATP-dependent DNA helicase II 70 kDa subunit antibody
      • CTC box binding factor 75 kDa subunit antibody
      • CTC box-binding factor 75 kDa subunit antibody
      • CTC75 antibody
      • CTCBF antibody
      • DNA repair protein XRCC6 antibody
      • G22P1 antibody
      • Ku 70 antibody
      • Ku autoantigen p70 subunit antibody
      • Ku autoantigen, 70kDa antibody
      • Ku p70 antibody
      • Ku70 antibody
      • Ku70 DNA binding component of DNA-dependent proteinkinase complex (thyroid autoantigen 70 kDa antibody
      • Kup70 antibody
      • Lupus Ku autoantigen protein p70 antibody
      • ML8 antibody
      • Thyroid autoantigen 70kD (Ku antigen) antibody
      • Thyroid autoantigen antibody
      • Thyroid lupus autoantigen antibody
      • Thyroid lupus autoantigen p70 antibody
      • Thyroid-lupus autoantigen antibody
      • TLAA antibody
      • X ray repair complementing defective repair in Chinese hamster cells 6 antibody
      • X-ray repair complementing defective repair in Chinese hamster cells 6 antibody
      • X-ray repair cross-complementing protein 6 antibody
      • XRCC 6 antibody
      • Xrcc6 antibody
      • XRCC6_HUMAN antibody
      see all

    Anti-Ku70 antibody [EPR4026] 图像

    • All lanes : Anti-Ku70 antibody [EPR4026] (ab108604) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : A673 cell lysate
      Lane 5 : A549 cell lysate
      Lane 6 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 70 kDa
      Observed band size : 70 kDa
    • ab108604, at 1/100 dilution, staining Ku70 in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.
    • ab108604, at 1/100 dilution, staining Ku70 in HeLa cells by Immunofluorescence.

    Anti-Ku70 antibody [EPR4026] (ab108604)参考文献

    ab108604 has not yet been referenced specifically in any publications.

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    Thank you for contacting us.
    We tested this antibody against various mouse samples in WB (e.g. heart, kidney, brain, spleen).

    In all cases, we didn’t see any specific signals or bands for the target protein. Therefore, we didn&rsquo...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"