概述

  • 产品名称Anti-Ku70抗体
    参阅全部 Ku70 一抗
  • 描述
    小鼠单克隆抗体to Ku70
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-610 of Human Ku70

  • 阳性对照
    • This antibody gave a positive result when used in the following methanol fixed cell lines: MALME-3M

性能

应用

Our Abpromise guarantee covers the use of ab58150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
  • 应用说明WB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
    • 序列相似性Belongs to the ku70 family.
      Contains 1 Ku domain.
      Contains 1 SAP domain.
    • 发展阶段Expression does not increase during promyelocyte differentiation.
    • 翻译后修饰Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair.
    • 细胞定位Nucleus. Chromosome.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 5''-deoxyribose-5-phosphate lyase Ku70 antibody
      • 5''-dRP lyase Ku70 antibody
      • 70 kDa subunit of Ku antigen antibody
      • ATP dependent DNA helicase 2 subunit 1 antibody
      • ATP dependent DNA helicase II 70 kDa subunit antibody
      • ATP-dependent DNA helicase 2 subunit 1 antibody
      • ATP-dependent DNA helicase II 70 kDa subunit antibody
      • CTC box binding factor 75 kDa subunit antibody
      • CTC box-binding factor 75 kDa subunit antibody
      • CTC75 antibody
      • CTCBF antibody
      • DNA repair protein XRCC6 antibody
      • G22P1 antibody
      • Ku 70 antibody
      • Ku autoantigen p70 subunit antibody
      • Ku autoantigen, 70kDa antibody
      • Ku p70 antibody
      • Ku70 antibody
      • Ku70 DNA binding component of DNA-dependent proteinkinase complex (thyroid autoantigen 70 kDa antibody
      • Kup70 antibody
      • Lupus Ku autoantigen protein p70 antibody
      • ML8 antibody
      • Thyroid autoantigen 70kD (Ku antigen) antibody
      • Thyroid autoantigen antibody
      • Thyroid lupus autoantigen antibody
      • Thyroid lupus autoantigen p70 antibody
      • Thyroid-lupus autoantigen antibody
      • TLAA antibody
      • X ray repair complementing defective repair in Chinese hamster cells 6 antibody
      • X-ray repair complementing defective repair in Chinese hamster cells 6 antibody
      • X-ray repair cross-complementing protein 6 antibody
      • XRCC 6 antibody
      • Xrcc6 antibody
      • XRCC6_HUMAN antibody
      see all

    Anti-Ku70 antibody 图像

    • ICC/IF image of ab58150 stained MALME-3M cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab58150 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Ku70 antibody (ab58150) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human breast cancer.


    • Predicted band size : 70 kDa
      Ku70 antibody (ab58150) at 1ug/lane + C32 cell lysate at 25ug/lane.

    Anti-Ku70 antibody (ab58150)参考文献

    This product has been referenced in:
    • Thong AE  et al. Tissue slice grafts of human renal cell carcinoma: An authentic preclinical model with high engraftment rate and metastatic potential. Urol Oncol N/A:N/A (2013). Read more (PubMed: 23911681) »
    • Zheng Y  et al. Host Protein Ku70 Binds and Protects HIV-1 Integrase from Proteasomal Degradation and Is Required for HIV Replication. J Biol Chem 286:17722-35 (2011). WB ; Human . Read more (PubMed: 21454661) »

    See all 2 Publications for this product

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