Anti-Ku70 (acetyl K331)抗体(ab190626)
Key features and details
- Rabbit polyclonal to Ku70 (acetyl K331)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-Ku70 (acetyl K331)抗体
参阅全部 Ku70 一抗 -
描述
兔多克隆抗体to Ku70 (acetyl K331) -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse
预测可用于: Rat, Human -
免疫原
Synthetic peptide within Human Ku70 aa 250-350 (acetyl K331). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Synthesized acetyl-peptide derived from human Ku70 around the acetylation site of Lys331.
Database link: P12956 -
阳性对照
- Extracts from 4T1 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 49% PBS
without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
The antibody against non-acetyl-peptide was removed by chromatography using non-acetyl-peptide corresponding to the acetylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab190626于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 70 kDa.
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说明 |
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WB
1/500 - 1/1000. Predicted molecular weight: 70 kDa. |
靶标
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功能
Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. -
序列相似性
Belongs to the ku70 family.
Contains 1 Ku domain.
Contains 1 SAP domain. -
发展阶段
Expression does not increase during promyelocyte differentiation. -
翻译后修饰
Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair. -
细胞定位
Nucleus. Chromosome. - Information by UniProt
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数据库链接
- Entrez Gene: 2547 Human
- Entrez Gene: 14375 Mouse
- Entrez Gene: 25019 Rat
- Omim: 152690 Human
- SwissProt: P12956 Human
- SwissProt: P23475 Mouse
- Unigene: 292493 Human
- Unigene: 288809 Mouse
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别名
- 5''-deoxyribose-5-phosphate lyase Ku70 antibody
- 5''-dRP lyase Ku70 antibody
- 70 kDa subunit of Ku antigen antibody
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab190626 尚未被引用在任何文献中。