概述

  • 产品名称Anti-KSR2抗体
    参阅全部 KSR2 一抗
  • 描述
    兔多克隆抗体to KSR2
  • 特异性Detects endogenous levels of total KSR2 protein.
  • 经测试应用适用于: WB, ELISA, ICC/IF, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide from an internal region of human KSR2

  • 阳性对照
    • WB: 293 cell extract ICC/IF: HeLa cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab72753 was Affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab72753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).
ELISA 1/40000.
ICC/IF 1/500 - 1/1000.
IP Use a concentration of 5 µg/ml.

靶标

  • 功能Location-regulated scaffold connecting MEK to RAF. Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production.
  • 组织特异性Mainly expressed in brain and kidney.
  • 序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
  • 结构域The protein kinase domain is predicted to be catalytically inactive.
  • 翻译后修饰Phosphorylated on Ser-474 by MARK3.
  • 细胞定位Cytoplasm. Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ25965 antibody
    • hKSR 2 antibody
    • hKSR2 antibody
    • Kinase suppressor of Ras 2 antibody
    • KSR 2 antibody
    • Ksr2 antibody
    • KSR2_HUMAN antibody
    see all

Anti-KSR2 antibody 图像

  • All lanes : Anti-KSR2 antibody (ab72753) at 1/500 dilution

    Lane 1 : 293 cell extract
    Lane 2 : 293 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 108 kDa
    Observed band size : 108 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of KSR2 in HeLa cells, using ab72753 at 1/500 dilution. Right panel was also treated with immunising peptide.
  • KSR2 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 5µg of Rabbit polyclonal to KSR2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab72753.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 108kDa; KSR2

Anti-KSR2 antibody (ab72753)参考文献

ab72753 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab72753.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"