Anti-KSR2抗体(ab72753)
Key features and details
- Rabbit polyclonal to KSR2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-KSR2抗体
参阅全部 KSR2 一抗 -
描述
兔多克隆抗体to KSR2 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide from an internal region of human KSR2
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab72753 was Affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72753于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).
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ICC/IF |
1/500 - 1/1000.
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa). |
ICC/IF
1/500 - 1/1000. |
靶标
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功能
Location-regulated scaffold connecting MEK to RAF. Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production. -
组织特异性
Mainly expressed in brain and kidney. -
序列相似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain. -
结构域
The protein kinase domain is predicted to be catalytically inactive. -
翻译后修饰
Phosphorylated on Ser-474 by MARK3. -
细胞定位
Cytoplasm. Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 283455 Human
- Entrez Gene: 333050 Mouse
- Omim: 610737 Human
- SwissProt: Q6VAB6 Human
- SwissProt: Q3UVC0 Mouse
- Unigene: 375836 Human
- Unigene: 437947 Mouse
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别名
- FLJ25965 antibody
- hKSR 2 antibody
- hKSR2 antibody
see all
图片
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All lanes : Anti-KSR2 antibody (ab72753) at 1/500 dilution
Lane 1 : 293 cell extract
Lane 2 : 293 cell extract with immunising peptide at 10 µg
Lysates/proteins at 10 µg per lane.
Predicted band size: 108 kDa
Observed band size: 108 kDa
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands. -
Immunofluorescence analysis of KSR2 in HeLa cells, using ab72753 at 1/500 dilution. Right panel was also treated with immunising peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab72753 被引用在 2 文献中.
- Jungfleisch J et al. CHIKV infection reprograms codon optimality to favor viral RNA translation by altering the tRNA epitranscriptome. Nat Commun 13:4725 (2022). PubMed: 35953468
- Castellanos-Martín A et al. Unraveling heterogeneous susceptibility and the evolution of breast cancer using a systems biology approach. Genome Biol 16:40 (2015). PubMed: 25853295