重组Anti-KSR1抗体[EPR2421Y] (ab68483)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2421Y] to KSR1
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-KSR1抗体[EPR2421Y]
参阅全部 KSR1 一抗 -
描述
兔单克隆抗体[EPR2421Y] to KSR1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Wild-type HAP1 cell lysate. HeLa, MEF, Rat1 and HEK-293 cell lysate. IHC-P: Human cervical carcinoma tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2421Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab68483于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/2000. Detects a band of approximately 120 kDa (predicted molecular weight: 102 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse and rat samples. |
说明 |
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WB
1/500 - 1/2000. Detects a band of approximately 120 kDa (predicted molecular weight: 102 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse and rat samples. |
靶标
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功能
Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity. -
序列相似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated. -
细胞定位
Cytoplasm. Membrane. In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery. - Information by UniProt
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数据库链接
- Entrez Gene: 8844 Human
- Entrez Gene: 16706 Mouse
- Entrez Gene: 360573 Rat
- Omim: 601132 Human
- SwissProt: Q8IVT5 Human
- SwissProt: Q61097 Mouse
- Unigene: 133534 Human
- Unigene: 4745 Mouse
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别名
- BKSR 1 antibody
- BKSR1 antibody
- dKsr antibody
see all
图片
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Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: KSR1 knockout HAP1 cell lysate (40 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: HEK293 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab68483 observed at 120 kDa. Red - loading control, ab8245, observed at 37 kDa.Unpurified ab68483 was shown to recognize KSR1 when KSR1 knockout samples were used, along with additional cross-reactive bands. Wild-type and KSR1 knockout samples were subjected to SDS-PAGE. Ab68483 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue sections labeling KSR1 with Purified ab68483 at 1:100 dilution (1.49 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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Anti-KSR1 antibody [EPR2421Y] (ab68483) at 1/1000 dilution (purified) + MEF (Mouse embryonic fibroblast (immortalized)) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 102 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST
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Anti-KSR1 antibody [EPR2421Y] (ab68483) at 1/1000 dilution (purified) + HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 102 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST
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Anti-KSR1 antibody [EPR2421Y] (ab68483) at 1/1000 dilution (purified) + Rat1 (Rat embryonic fibroblast) whole cell lysates
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 102 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST
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Anti-KSR1 antibody [EPR2421Y] (ab68483) at 1/2000 dilution (unpurified) + HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate at 10 µg
Secondary
goat anti rabbit HRP at 1/2000 dilution
Predicted band size: 102 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab68483 被引用在 4 文献中.
- Rao C et al. KSR1- and ERK-dependent translational regulation of the epithelial-to-mesenchymal transition. Elife 10:N/A (2021). PubMed: 33970103
- Parvathaneni S et al. Calmodulin influences MAPK signaling by binding KSR1. J Biol Chem 296:100577 (2021). PubMed: 33766558
- Lavoie H et al. MEK drives BRAF activation through allosteric control of KSR proteins. Nature 554:549-553 (2018). PubMed: 29433126
- Bai Y et al. Effect of lentiviral vector-mediated KSR1 gene silencing on the proliferation of renal tubular epithelial cells and expression of inflammatory factors in a rat model of ischemia/reperfusion injury. Acta Biochim Biophys Sin (Shanghai) 50:807-816 (2018). PubMed: 30020400