概述

  • 产品名称
  • 描述
    兔多克隆抗体to KSR1
  • 特异性
    This antibody detects endogenous levels of total KSR protein.
  • 经测试应用
    适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A synthetic non-phosphopeptide derived from human KSR around the phosphorylation site of serine 392.

  • 阳性对照
    • Human brain tissue and extracts from NIH/3T3 cells

性能

应用

Our Abpromise guarantee covers the use of ab52196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
IHC-P
  • 应用说明
    ELISA: 1/20000.
    IHC-P: 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    WB: 1/500 - 1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity.
    • 序列相似性
      Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 protein kinase domain.
    • 翻译后修饰
      Phosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated.
    • 细胞定位
      Cytoplasm. Membrane. In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BKSR 1 antibody
      • BKSR1 antibody
      • dKsr antibody
      • EK 31 antibody
      • EK31 antibody
      • hb antibody
      • Kinase suppressor of Ras 1 antibody
      • Kinase suppressor of ras antibody
      • KSR 1 antibody
      • KSR antibody
      • KSR1 antibody
      • KSR1_HUMAN antibody
      • Positive Ras signaling mediator family member (86.4 kD) antibody
      • Positive Ras signaling mediator family member antibody
      • RSU 2 antibody
      • RSU2 antibody
      • SR 31 antibody
      • SR31 antibody
      • Suppressor of activated let 60 Ras SUR3 antibody
      • Suppressor of Ras1 31 antibody
      see all

    图片

    • Immunohistochemical analysis of paraffin-embedded human brain tissue using ab52196 at a 1/50 dilution.

      Left image un-treated.

      Right image treated with peptide.

    • All lanes : Anti-KSR1 antibody (ab52196) at 1/500 dilution

      Lane 1 : Extracts from NIH/3T3 cells treated with PDGF (50ng/ml, 20min).
      Lane 2 : Extracts from NIH/3T3 cells treated with PDGF (50ng/ml, 20min) and peptide.


      Predicted band size : 102 kDa
      Observed band size : 102 kDa

    文献

    This product has been referenced in:
    • Turski ML  et al. A novel role for copper in Ras/mitogen-activated protein kinase signaling. Mol Cell Biol 32:1284-95 (2012). WB ; Mouse . Read more (PubMed: 22290441) »
    • Thompson T  et al. 1,25-dihydroxyvitamin D3 enhances the apoptotic activity of MDM2 antagonist nutlin-3a in acute myeloid leukemia cells expressing wild-type p53. Mol Cancer Ther 9:1158-68 (2010). WB ; Human . Read more (PubMed: 20406950) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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