Mouse, Rat, Cow
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 175 and 381 of Human KRT31 (UniProt ID: Q15323).
HeLa whole cell lysate; HeLa cells
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
1/100 - 1/500.
Present in scalp but not in hairless skin. Abundantly expressed in the differentiating cortex of growing (anagen) hair. Expression is restricted to the keratinocytes of the hair cortex and is absent from inner root sheath and medulla.
Belongs to the intermediate filament family.
Information by UniProt
Hair Keratin acidic hHa1 antibody
Western blot - Anti-KRT31 antibody (ab111730)
Anti-KRT31 antibody (ab111730) at 1/5000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 47 kDa 10% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-KRT31 antibody (ab111730)
Immunofluorescence analysis of KRT31 in methanol-fixed HeLa cells, using ab111730 at a 1/200 dilution. The lower image shows the cells counter stained with Hoechst 33342.
has not yet been referenced specifically in any publications.
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