Anti-KPNA6抗体(ab82149)

概述

  • 产品名称Anti-KPNA6抗体
    参阅全部 KPNA6 一抗
  • 描述
    山羊多克隆抗体to KPNA6
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow, Dog, Pig, Cynomolgus Monkey, Orangutan
  • 免疫原

    Peptide with sequence

    C-STTGESVITREMVE

    , corresponding to internal sequence amino acids 74-87 of human KPNA6 (NP_036448.1).

  • 阳性对照
    • Human skeletal muscle lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab82149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 60 kDa. A customer reported that milk can significantly suppress antigen-antibody interaction on nitrocellulose membrane in WB.
IHC-P Use a concentration of 3 - 6 µg/ml.

靶标

  • 功能Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
  • 组织特异性Widely expressed.
  • 序列相似性Belongs to the importin alpha family.
    Contains 10 ARM repeats.
    Contains 1 IBB domain.
  • Information by UniProt
  • 数据库链接
  • 形式Cellular location: cytoplasm; nucleus.
  • 别名
    • FLJ11249 antibody
    • IMA7_HUMAN antibody
    • Importin alpha 7 subunit antibody
    • Importin alpha S2 antibody
    • Importin subunit alpha 7 antibody
    • Importin subunit alpha-7 antibody
    • IPOA 7 antibody
    • IPOA7 antibody
    • Karyopherin alpha 6 antibody
    • Karyopherin alpha 6 (importin alpha 7) antibody
    • Karyopherin subunit alpha 6 antibody
    • Karyopherin subunit alpha-6 antibody
    • KPNA 6 antibody
    • KPNA 7 antibody
    • Kpna6 antibody
    • KPNA7 antibody
    • MGC17918 antibody
    • OTTHUMP00000004532 antibody
    see all

Anti-KPNA6 antibody 图像

  • Anti-KPNA6 antibody (ab82149) at 0.1 µg/ml + human skeletal muscle lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 60 kDa
    Observed band size : 60 kDa


    Exposure time : 1 hour

    Some minor background is detected and is blocked by the immunizing peptide.
  • ab82149 at 3.8 µg/ml staining KPNA6 in Human adrenal gland tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-KPNA6 antibody (ab82149)参考文献

ab82149 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"