概述

  • 产品名称Anti-KPNA5抗体
    参阅全部 KPNA5 一抗
  • 描述
    兔多克隆抗体to KPNA5
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to 18 amino acids near the N terminal of Human KPNA5.

  • 阳性对照
    • EL4 cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab125529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 60 kDa.
ICC/IF Use a concentration of 20 µg/ml.

靶标

  • 功能Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates nuclear import of STAT1 homodimers and STAT1/STAT2 heterodimers by recognizing non-classical NLSs of STAT1 and STAT2 through ARM repeats 8-9. Recognizes influenza A virus nucleoprotein through ARM repeat 7-9 In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.
  • 组织特异性Testis.
  • 序列相似性Belongs to the importin alpha family.
    Contains 10 ARM repeats.
    Contains 1 IBB domain.
  • 结构域Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
    The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
    The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • IMA5_HUMAN antibody
    • Importin alpha 6 antibody
    • Importin subunit alpha 6 antibody
    • Importin subunit alpha-6 antibody
    • IPOA 6 antibody
    • IPOA6 antibody
    • Karyopherin alpha 5 antibody
    • Karyopherin alpha 5 importin alpha 6 antibody
    • Karyopherin subunit alpha 5 antibody
    • Karyopherin subunit alpha-5 antibody
    • KPNA 5 antibody
    • KPNA5 antibody
    • SRP 6 antibody
    • SRP6 antibody
    see all

Anti-KPNA5 antibody 图像

  • Immunofluorescence of KPNA5 in EL4 cells with ab125529 at 20 ug/mL.
  • Immunocytochemistry of KPNA5 in EL4 cells with ab125529 at 5 μg/mL.

  • All lanes : Anti-KPNA5 antibody (ab125529) at 1 µg/ml

    Lane 1 : EL4 cell lysate
    Lane 2 : EL4 cell lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 60 kDa

Anti-KPNA5 antibody (ab125529)参考文献

ab125529 has not yet been referenced specifically in any publications.

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