概述

  • 产品名称Anti-KPNA4抗体
    参阅全部 KPNA4 一抗
  • 描述
    山羊多克隆抗体to KPNA4
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow, Dog
  • 免疫原

    Peptide with sequence NSSANVPTEGFQF, from C Terminus of the protein sequence according to NP_002259

  • 阳性对照
    • Human testis and ovary lysate. Human testis tissue sections and human small intestine sections.
  • 常规说明GenBank Accession Number – NP_002259.


    The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody说明The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab6039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 - 2 µg/ml.
WB Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 55-60 kDa (predicted molecular weight: 58 kDa).Can be blocked with Human KPNA4 peptide (ab23144). A customer reported that milk can significantly suppress antigen-antibody interaction on nitrocellulose membrane in WB.

靶标

  • 功能Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.
  • 组织特异性Highly expressed in testis, ovary, small intestine, heart, skeletal muscle, lung and pancreas, but barely detectable in kidney, thymus, colon and peripheral blood leukocytes.
  • 序列相似性Belongs to the importin alpha family.
    Contains 10 ARM repeats.
    Contains 1 IBB domain.
  • 结构域Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
    The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
    The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ31113 antibody
    • IMA3_HUMAN antibody
    • Importin alpha 3 antibody
    • Importin alpha 4 subunit antibody
    • Importin alpha Q1 antibody
    • Importin subunit alpha 3 antibody
    • Importin subunit alpha-3 antibody
    • IPO A3 antibody
    • IPOA 3 antibody
    • IPOA3 antibody
    • Karyopherin alpha 4 (importin alpha 3) antibody
    • Karyopherin alpha 4 antibody
    • Karyopherin alpha 4 importin alpha 3 antibody
    • Karyopherin alpha 4 subunit antibody
    • Karyopherin subunit alpha 4 antibody
    • Karyopherin subunit alpha-4 antibody
    • KPNA 4 antibody
    • Kpna4 antibody
    • MGC12217 antibody
    • MGC26703 antibody
    • OTTHUMP00000213880 antibody
    • QIP 1 antibody
    • Qip1 antibody
    • Qip1 protein antibody
    • SRP 3 antibody
    • SRP3 antibody
    see all

Anti-KPNA4 antibody 图像

  • Immunohistochemical analysis of 10% buffered formalin-fixed paraffin-embedded murine nephric tissue sections, labelling KPNA4 with ab6039 at  a dilution of 1/100 incubated for 12 hours at 4°C. Heat mediated antigen retrival was performed through 10mM citrate buffer at pH 6.0. Blocking was with 5% serum incubated for 1 hour at 21°C. Secondary was a polyclonal Donkey anti-Goat Alexa Fluor® 647 conjugate at 1/200. Counterstain is DAPI against nuclear DNA. The Negative control is the same as above, but in the abscence of the primary ab6039.

    See Abreview



  • Predicted band size : 58 kDa
    ab6039 at 0.5µg/ml staining approximately 55-60kDa band of KPNA4 in Human Testis lysate by Western blot (ECL). ab6039 at 0.5µg/ml staining approximately 55-60kDa band of KPNA4 in Human Testis lysate by Western blot (ECL).
  • ab6039 (3µg/ml) staining Human Testis by IHC-P. Microwaved antigen retrieval with citrate buffer (pH 6), HRP-staining.
  • ab6039 at 5µg/ml staining KPNA4 in human small intestinal tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-KPNA4 antibody (ab6039)参考文献

This product has been referenced in:
  • Audsley MD  et al. Nucleocytoplasmic trafficking of Nipah virus W protein involves multiple discrete interactions with the nuclear import and export machinery. Biochem Biophys Res Commun 479:429-433 (2016). IP ; Human . Read more (PubMed: 27622322) »
  • De Houwer S  et al. The HIV-1 integrase mutant R263A/K264A is 2-fold defective for TRN-SR2 binding and viral nuclear import. J Biol Chem 289:25351-61 (2014). WB ; Human . Read more (PubMed: 25063804) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (kidney)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer pH6
Permeabilization No
Specification kidney
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% buffered formalin
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Abcam user community

Verified customer

提交于 Nov 11 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (pancreatic tumor)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate buffer pH6
Permeabilization No
Specification pancreatic tumor
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% buffered formalin
Username

Abcam user community

Verified customer

提交于 Oct 29 2015

I hope that you are well.

I am sending this email to inquire about the updates you might have about the WB analysis. There were few anti KPNA4 products that were proving difficult to use and as per your last email you were suppose to contact m...

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Thank you for your email.

Two SRP1 antibodies failed, which is very strange. We have used SRP1 transfected 293T cells line in the characterization of antibody ab124646, this means that the 293T cells seems not expressing the protein. I would ...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HCT116, HT29 and Caco2 colon cancer cell lines)
Loading amount 30 µg
Specification HCT116, HT29 and Caco2 colon cancer cell lines
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% in TBST
Username

Abcam user community

Verified customer

提交于 Nov 07 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"