概述

  • 产品名称Anti-KPNA3抗体
    参阅全部 KPNA3 一抗
  • 描述
    山羊多克隆抗体to KPNA3
  • 经测试应用适用于: IHC-P, IP, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
    预测可用于: Dog
  • 免疫原

    Synthetic peptide: DPTANLQTKEFNF, corresponding to C terminal amino acids 509-521 of Human KPNA3.

  • 阳性对照
    • Human testis and kidney lysate. Human heart tissue sections.
  • 常规说明GenBank Accession Number – NP_002258.


    The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA3, encodes a protein similar to certain nuclear transport proteins of Xenopus and human. The predicted amino acid sequence shows similarity to Xenopus importin, yeast SRP1, and human RCH1 (KPNA2), respectively. The similarities among these proteins suggests that karyopherin alpha-3 may be involved in the nuclear transport system.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody说明The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA3, encodes a protein similar to certain nuclear transport proteins of Xenopus and human. The predicted amino acid sequence shows similarity to Xenopus importin, yeast SRP1, and human RCH1 (KPNA2), respectively. The similarities among these proteins suggests that karyopherin alpha-3 may be involved in the nuclear transport system.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab6038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 - 10 µg/ml.
IP Use at an assay dependent concentration. PubMed: 20454918
ICC/IF Use at an assay dependent concentration. PubMed: 21291862
WB Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 55-60 kDa (predicted molecular weight: 58 kDa).Can be blocked with Human KPNA3 peptide (ab23142).

靶标

  • 功能Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. Recognizes NLSs of influenza A virus nucleoprotein probably through ARM repeats 7-9.
  • 组织特异性Ubiquitous. Highest levels in heart and skeletal muscle.
  • 序列相似性Belongs to the importin alpha family.
    Contains 10 ARM repeats.
    Contains 1 IBB domain.
  • 结构域Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
    The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
    The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • SRP1 gamma antibody
    • hSRP1 antibody
    • IMA4_HUMAN antibody
    • Importin alpha 3 antibody
    • Importin alpha 3 subunit antibody
    • Importin alpha 4 antibody
    • Importin alpha Q2 antibody
    • Importin subunit alpha-4 antibody
    • IPOA4 antibody
    • Karyopherin alpha 3 antibody
    • Karyopherin alpha 3 importin alpha 4 antibody
    • Karyopherin alpha 3 subunit antibody
    • Karyopherin subunit alpha 3 antibody
    • Karyopherin subunit alpha-3 antibody
    • KPNA 3 antibody
    • Kpna3 antibody
    • Qip2 antibody
    • SRP1 antibody
    • SRP1-gamma antibody
    • SRP1gamma antibody
    • SRP4 antibody
    see all

Anti-KPNA3 antibody 图像



  • Predicted band size : 58 kDa


    ab6038 at 0.1µg/ml staining approximately 55-60kDa band of KPNA3 in Human Testis Lysate by Western blot (ECL).
  • ab6038 at 5ug/ml staining KPNA3 in human heart tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.

Anti-KPNA3 antibody (ab6038)参考文献

This product has been referenced in:
  • Baker SA  et al. Karyopherin a 3 and karyopherin a 4 proteins mediate the nuclear import of methyl-CpG binding protein 2. J Biol Chem 290:22485-93 (2015). Read more (PubMed: 26245896) »
  • Lieu KG  et al. The p53-induced factor Ei24 inhibits nuclear import through an importin ß-binding-like domain. J Cell Biol 205:301-12 (2014). Read more (PubMed: 24821838) »

See all 9 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab6038.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"