概述

  • 产品名称Anti-KPNA3抗体
    参阅全部 KPNA3 一抗
  • 描述
    山羊多克隆抗体to KPNA3
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Chicken, Cow, Dog, Pig, Rhesus monkey
  • 免疫原

    Synthetic peptide:

    DPTANLQTKEFNF

    with a Cysteine residue linker, corresponding to C terminal amino acids 509 - 521 of Human KPNA3.

  • 阳性对照
    • Human heart tissue; Human testis lysate.

性能

应用

Our Abpromise guarantee covers the use of ab115209 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 58 kDa.
ELISA Use at an assay dependent dilution.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. Recognizes NLSs of influenza A virus nucleoprotein probably through ARM repeats 7-9.
  • 组织特异性Ubiquitous. Highest levels in heart and skeletal muscle.
  • 序列相似性Belongs to the importin alpha family.
    Contains 10 ARM repeats.
    Contains 1 IBB domain.
  • 结构域Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
    The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
    The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • SRP1 gamma antibody
    • hSRP1 antibody
    • IMA4_HUMAN antibody
    • Importin alpha 3 antibody
    • Importin alpha 3 subunit antibody
    • Importin alpha 4 antibody
    • Importin alpha Q2 antibody
    • Importin subunit alpha-4 antibody
    • IPOA4 antibody
    • Karyopherin alpha 3 antibody
    • Karyopherin alpha 3 importin alpha 4 antibody
    • Karyopherin alpha 3 subunit antibody
    • Karyopherin subunit alpha 3 antibody
    • Karyopherin subunit alpha-3 antibody
    • KPNA 3 antibody
    • Kpna3 antibody
    • Qip2 antibody
    • SRP1 antibody
    • SRP1-gamma antibody
    • SRP1gamma antibody
    • SRP4 antibody
    see all

Anti-KPNA3 antibody 图像

  • ab115209 at 5 ug/ml, staining KPNA3 in Formalin-fixed, Paraffin-embedded Human heart tissue by immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-KPNA3 antibody (ab115209) at 0.1 µg/ml + Human testis lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 58 kDa

Anti-KPNA3 antibody (ab115209)参考文献

ab115209 has not yet been referenced specifically in any publications.

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