- 数据表
- 具体的参考文献 (3)
- 实验方案
概述
- 产品名称Anti-KMT8/Riz1/Riz2抗体
- 描述兔多克隆抗体to KMT8/Riz1/Riz2
- 特异性This antibody recognizes the beta isoform (Riz 2) of PRDM 2 (185 kDa) as well as RIZ 1. It will not detect isoform 4.
- 经测试应用适用于: IP, WB, IHC-Pmore details
- 种属反应性与反应: Mouse, Rat, Human
- 免疫原
Fusion protein (GST-tag) corresponding to Rat KMT8/Riz1/Riz2 aa 245-573.
- 阳性对照
- IP: Rat B50 brain tumour cell lysate.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- 存储溶液Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... - 纯度Protein A purified
- 纯化说明This antibody is purified through a protein A column, followed by peptide affinity purification.
- Primary antibody说明Similar to acetylation and phosphorylation, histone methylation at the N-terminal tail has emerged as an important role in regulating chromatin dynamics and gene activity. Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET-domain-containing methyltransferase family. Five members have been identified in the arginine methyltransferase family. About 27 are grouped into the SET-domain family, and another 17 make up the PR domain family that is related to the SET domain family. The retinoblastoma protein-interacting zinc finger gene RIZ 1 is a tumor suppressor gene and a founding member of the PR domain family. RIZ 1 inactivation is commonly found in many types of human cancers and occurs through loss of mRNA expression, frame shift mutation, chromosomal deletion, and missense mutation. RIZ 1 is also a tumor susceptibility gene in mice. The loss of RIZ 1 mRNA in human cancers was shown to associate with DNA methylation of its promoter CpG island. Methylation of the RIZ 1 promoter strongly correlated with lost or decreased RIZ 1 mRNA expression in breast, liver, colon, and lung cancer cell lines as well as in liver cancer tissues.
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab3790 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 189 kDa. PubMed: 24423864 | |
| IHC-P | 1/100 - 1/500. |
靶标
- 功能S-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3. May function as a DNA-binding transcription factor. Binds to the macrophage-specific TPA-responsive element (MTE) of the HMOX1 (heme oxygenase 1) gene and may act as a transcriptional activator of this gene.
- 组织特异性Highly expressed in retinoblastoma cell lines and in brain tumors. Also expressed in a number of other cell lines and in brain, heart, skeletal muscle, liver and spleen. Isoform 1 is expressed in testis at much higher level than isoform 3.
- 序列相似性Contains 8 C2H2-type zinc fingers.
Contains 1 SET domain. - 细胞定位Nucleus.
- Information by UniProt
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数据库链接
- Entrez Gene: 7799 Human
- Entrez Gene: 110593 Mouse
- Entrez Gene: 313678 Rat
- Omim: 601196 Human
- SwissProt: Q13029 Human
- SwissProt: Q63755 Rat
- Unigene: 371823 Human
- Unigene: 202632 Rat
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别名
- GATA 3 binding protein G3B antibody
- GATA-3-binding protein G3B antibody
- HUMHOXY1 antibody
see all
Anti-KMT8/Riz1/Riz2 antibody 图像
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Immunoprecipitation - Anti-KMT8/Riz1/Riz2 antibody (ab3790)Rat B50 brain tumor cell extracts were immunoprecipitated with RIZ antibody or preimmune (Liu L. et al. J. Biol. Chem. 1997. 272: 2984.).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT8/Riz1/Riz2 antibody (ab3790)Immunohistochemical analysis of RIZ1 in adenovirus-transduced tumor cells. Established HCT116 xenograft tumors at day 2 after infection of AdRIZ1, Adnull, or buffer alone were processed for RIZ1 in situ detection (TUNEL) analysis (Jiang G-L. and Huang S. Cancer Res. 2001. 61: 1796.)
Anti-KMT8/Riz1/Riz2 antibody (ab3790)参考文献
This product has been referenced in:
- Cheedipudi S et al. A fine balance: epigenetic control of cellular quiescence by the tumor suppressor PRDM2/RIZ at a bivalent domain in the cyclin a gene. Nucleic Acids Res 43:6236-56 (2015). IHC-P ; Mouse . Read more (PubMed: 26040698) »
- Congdon LM et al. The PR-Set7 binding domain of Riz1 is required for the H4K20me1-H3K9me1 trans-tail 'histone code' and Riz1 tumor suppressor function. Nucleic Acids Res 42:3580-9 (2014). WB ; Human . Read more (PubMed: 24423864) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"