Anti-KMT6 / EZH2 (phospho S21)抗体(ab84989)

概述

  • 产品名称Anti-KMT6 / EZH2 (phospho S21)抗体
    参阅全部 KMT6 / EZH2 一抗
  • 描述
    兔多克隆抗体to KMT6 / EZH2 (phospho S21)
  • 经测试应用适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Phosphorylated synthetic peptide surrounding Serine 21 of human KMT6/ EZH2 (NP_004447.2)

  • 阳性对照
    • Human Breast Carcinoma, Colon Carcinoma, Mouse Teratoma.
  • 常规说明

    Concentration is optimized for IHC and not determined

性能

应用

Our Abpromise guarantee covers the use of ab84989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/500.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes.
  • 组织特异性Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis.
  • 序列相似性Belongs to the histone-lysine methyltransferase family. EZ subfamily.
    Contains 1 SET domain.
  • 发展阶段Expression decreases during senescence of embryonic fibroblasts (HEFs). Expression peaks at the G1/S phase boundary.
  • 翻译后修饰Phosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Enhancer of zeste 2 antibody
    • enhancer of zeste 2 polycomb repressive complex 2 subunit antibody
    • Enhancer of zeste homolog 2 (Drosophila) antibody
    • Enhancer of zeste homolog 2 antibody
    • Enhancer of zeste, Drosophila, homolog 2 antibody
    • ENX 1 antibody
    • Enx 1h antibody
    • ENX-1 antibody
    • ENX1 antibody
    • Enx1h antibody
    • EZH 2 antibody
    • EZH1 antibody
    • EZH2 antibody
    • EZH2_HUMAN antibody
    • EZH2b antibody
    • Histone-lysine N-methyltransferase EZH2 antibody
    • KMT 6 antibody
    • KMT6 antibody
    • KMT6A antibody
    • Lysine N-methyltransferase 6 antibody
    • MGC9169 antibody
    • WVS antibody
    • WVS2 antibody
    see all

Anti-KMT6 / EZH2 (phospho S21) antibody 图像

  • ab84989 at 1/250 dilution staining KMT6 / EZH2 in serial sections of human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
    Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right).
  • ab84989 at 1/250 dilution staining KMT6 / EZH2 in serial sections of mouse teratoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
    Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right).
  • ab84989 at 1/250 dilution staining KMT6 / EZH2 in human colon carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1/100.
  • ICC/IF image of ab84989 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84989, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-KMT6 / EZH2 (phospho S21) antibody (ab84989)参考文献

ab84989 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (PC3)
Specification PC3
Treatment Wortmannin 1/2000 for 1 hour
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 26 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (human glioma cell line)
Loading amount 30 µg
Specification human glioma cell line
Treatment 0-100µg/ml EF-001(Symbol) for 24hr
Gel Running Conditions Reduced Denaturing (15 %)
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 May 06 2013

Thank you for contacting Abcam earlier today.
For both lots GR21293-6 & -7, the concentration is 0.1mg/ml.
If there is anything else I can help you with, please let me know.

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (human breast cancer cell lines)
Loading amount 50 µg
Specification human breast cancer cell lines
Treatment phosphotase inhibitor (NAF & Vanadate)
Gel Running Conditions Reduced Denaturing (8% SDS-PAGE)
Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Khun La Win Myint

Verified customer

提交于 Jul 21 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"