The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
H4 K20 HMTase
H4 K20 specific histone methyltransferase
Histone H4 K20 methyltransferase
Histone lysine N methyltransferase H4 lysine 20 specific
Histone-lysine N-methyltransferase SETD8
Lysine N-methyltransferase 5A
N-lysine methyltransferase SETD8
Pr SET 7
PR/SET domain containing protein 07
PR/SET domain containing protein 8
PR/SET domain-containing protein 07
SET domain containing (lysine methyltransferase) 8
SET domain containing 8
SET domain containing lysine methyltransferase 8
SET domain containing protein 8
SET domain-containing protein 8
功能Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes.
序列相似性Belongs to the histone-lysine methyltransferase family. PR/SET subfamily. Contains 1 SET domain.
发展阶段Not detected during G1 phase. First detected during S through G2 phases, and peaks during mitosis (at protein level).
结构域Although the SET domain contains the active site of enzymatic activity, both sequences upstream and downstream of the SET domain are required for methyltransferase activity.
细胞定位Nucleus. Chromosome. Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin.