Anti-KMT5A / SETD8 / Pr-SET7抗体(ab111691)

概述

  • 产品名称Anti-KMT5A / SETD8 / Pr-SET7抗体
    参阅全部 KMT5A / SETD8 / Pr-SET7 一抗
  • 描述
    兔多克隆抗体to KMT5A / SETD8 / Pr-SET7
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 81-290 of Human KMT5A/ SETD8/ Pr-SET7, isoform 1(42kda) and 2(39KDa)

  • 阳性对照
    • NT2D1, IMR32, U-87 MG and MCF7 whole cell lysates; HeLa xenograft tissue.

性能

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应用

Our Abpromise guarantee covers the use of ab111691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 39 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes.
  • 序列相似性Belongs to the histone-lysine methyltransferase family. PR/SET subfamily.
    Contains 1 SET domain.
  • 发展阶段Not detected during G1 phase. First detected during S through G2 phases, and peaks during mitosis (at protein level).
  • 结构域Although the SET domain contains the active site of enzymatic activity, both sequences upstream and downstream of the SET domain are required for methyltransferase activity.
  • 细胞定位Nucleus. Chromosome. Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin.
  • Information by UniProt
  • 数据库链接
  • 别名
    • H4 K20 HMTase antibody
    • H4 K20 specific histone methyltransferase antibody
    • H4-K20-HMTase SETD8 antibody
    • Histone H4 K20 methyltransferase antibody
    • Histone lysine N methyltransferase H4 lysine 20 specific antibody
    • Histone-lysine N-methyltransferase SETD8 antibody
    • Lysine N-methyltransferase 5A antibody
    • N-lysine methyltransferase SETD8 antibody
    • Pr SET 7 antibody
    • Pr SET7 antibody
    • PR-Set7 antibody
    • PR/SET domain containing protein 07 antibody
    • PR/SET domain containing protein 8 antibody
    • PR/SET domain-containing protein 07 antibody
    • PR/SET07 antibody
    • PrSET7 antibody
    • SET 07 antibody
    • SET 8 antibody
    • SET domain containing (lysine methyltransferase) 8 antibody
    • SET domain containing 8 antibody
    • SET domain containing lysine methyltransferase 8 antibody
    • SET domain containing protein 8 antibody
    • SET domain-containing protein 8 antibody
    • SET07 antibody
    • SET8 antibody
    • SETD 8 antibody
    • setd8 antibody
    • SETD8_HUMAN antibody
    see all

Anti-KMT5A / SETD8 / Pr-SET7 antibody 图像

  • Anti-KMT5A / SETD8 / Pr-SET7 antibody (ab111691) at 1/2000 dilution + U-87 MG whole cell lysate at 30 µg

    Predicted band size : 39 kDa
  • ab111691 at 1/500 dilution staining KMT5A / SETD8 / Pr-SET7 in Formalin-fixed, paraffin-embedded HeLa xenograft tissue by Immunohistochemistry.

Anti-KMT5A / SETD8 / Pr-SET7 antibody (ab111691)参考文献

ab111691 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Mouse Cell lysate - whole cell (fibroblast)
Specification fibroblast
Treatment siRNA for 48hrs
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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提交于 Jul 09 2013

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