Anti-KMT5A / SETD8 / Pr-SET7抗体(ab111691)
Key features and details
- Rabbit polyclonal to KMT5A / SETD8 / Pr-SET7
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-KMT5A / SETD8 / Pr-SET7抗体
参阅全部 KMT5A / SETD8 / Pr-SET7 一抗 -
描述
兔多克隆抗体to KMT5A / SETD8 / Pr-SET7 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human KMT5A/ SETD8/ Pr-SET7 aa 50-300. Recombinant fragment, corresponding to a region within amino acids 81-290 of Human KMT5A/ SETD8/ Pr-SET7, isoform 1(42kda) and 2(39KDa)
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阳性对照
- NT2D1, IMR32, U-87 MG and MCF7 whole cell lysates; HeLa xenograft tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab111691于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (3) |
1/500 - 1/3000. Predicted molecular weight: 39 kDa.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 39 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. -
序列相似性
Belongs to the histone-lysine methyltransferase family. PR/SET subfamily.
Contains 1 SET domain. -
发展阶段
Not detected during G1 phase. First detected during S through G2 phases, and peaks during mitosis (at protein level). -
结构域
Although the SET domain contains the active site of enzymatic activity, both sequences upstream and downstream of the SET domain are required for methyltransferase activity. -
细胞定位
Nucleus. Chromosome. Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin. - Information by UniProt
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数据库链接
- Entrez Gene: 387893 Human
- Omim: 607240 Human
- SwissProt: Q9NQR1 Human
- Unigene: 443735 Human
- Unigene: 572262 Human
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别名
- H4 K20 HMTase antibody
- H4 K20 specific histone methyltransferase antibody
- H4-K20-HMTase SETD8 antibody
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图片
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab111691 被引用在 3 文献中.
- Li J et al. LncRNA LINC00473 is involved in the progression of invasive pituitary adenoma by upregulating KMT5A via ceRNA-mediated miR-502-3p evasion. Cell Death Dis 12:580 (2021). PubMed: 34091587
- Wang K et al. Di-methylation of CD147-K234 Promotes the Progression of NSCLC by Enhancing Lactate Export. Cell Metab 33:160-173.e6 (2021). PubMed: 33406400
- Xu Y et al. MiRNA-340-5p mediates the functional and infiltrative promotion of tumor-infiltrating CD8+ T lymphocytes in human diffuse large B cell lymphoma. J Exp Clin Cancer Res 39:238 (2020). PubMed: 33168024