Anti-KMT3A / HYPB / HIF-1抗体(ab69836)

概述

  • 产品名称
    Anti-KMT3A / HYPB / HIF-1抗体
    参阅全部 KMT3A / HYPB / HIF-1 一抗
  • 描述
    兔多克隆抗体to KMT3A / HYPB / HIF-1
  • 经测试应用
    适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Cat, Dog
  • 免疫原

    A region within synthetic peptide: ELNEEIKQSH SFSLQTPCSK GSELRMINKN PEREKAGSPA PSNRLNDSPT, corresponding to N terminal amino acids 577-626 of Human KMT3A/ HYPB/ HIF-1

  • 阳性对照
    • HepG2 cell lysate. Human placenta.

性能

应用

Our Abpromise guarantee covers the use of ab69836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately >70 kDa (predicted molecular weight: 288 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 功能
    Histone methyltransferase that methylates 'Lys-36' of histone H3. H3 'Lys-36' methylation represents a specific tag for epigenetic transcriptional activation. Probably plays a role in chromatin structure modulation during elongation via its interaction with hyperphosphorylated POLR2A. Binds DNA at promoters. May also act as a transcription activator that binds to promoters. Binds to the promoters of adenovirus 12 E1A gene in case of infection, possibly leading to regulate its expression.
  • 组织特异性
    Ubiquitously expressed.
  • 序列相似性
    Belongs to the histone-lysine methyltransferase family. SET2 subfamily.
    Contains 1 AWS domain.
    Contains 1 post-SET domain.
    Contains 1 SET domain.
    Contains 1 WW domain.
  • 结构域
    The low charge region mediates the transcriptional activation activity.
  • 翻译后修饰
    May be automethylated.
  • 细胞定位
    Nucleus. Chromosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC 2.1.1.43 antibody
    • FLJ16420 antibody
    • FLJ22472 antibody
    • FLJ23184 antibody
    • FLJ45883 antibody
    • HBP231 antibody
    • HIF 1 antibody
    • HIF-1 antibody
    • HIF1 antibody
    • HIP-1 antibody
    • Histone lysine N methyltransferase SETD2 antibody
    • Histone-lysine N-methyltransferase SETD2 antibody
    • hSET2 antibody
    • HSPC069 antibody
    • Huntingtin interacting protein 1 antibody
    • Huntingtin interacting protein antibody
    • Huntingtin interacting protein B antibody
    • Huntingtin interacting protein HYPB antibody
    • Huntingtin yeast partner B antibody
    • Huntingtin-binding protein, 231-KD antibody
    • Huntingtin-interacting protein 1 antibody
    • Huntingtin-interacting protein B antibody
    • HYPB antibody
    • KIAA1732 antibody
    • KMT3A antibody
    • Lysine N methyltransferase 3A antibody
    • Lysine N-methyltransferase 3A antibody
    • p231HBP antibody
    • SET domain containing 2 antibody
    • SET domain-containing protein 2 antibody
    • SET2 antibody
    • SETD2 antibody
    • SETD2_HUMAN antibody
    see all

Anti-KMT3A / HYPB / HIF-1 antibody 图像

  • All lanes : Anti-KMT3A / HYPB / HIF-1 antibody (ab69836) at 1.25 µg/ml

    Lane 1 : HepG2 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Molecular weight marker

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 288 kDa
    Observed band size : >70 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin-embedded human placenta using ab69836 at 4 µg/ml. Positively labelled trophoblast cells are indicated with arrows.
  • ICC/IF image of ab69836 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69836, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-KMT3A / HYPB / HIF-1 antibody (ab69836)参考文献

This product has been referenced in:
  • Zhou W  et al. MicroRNA-138 inhibits hypoxia-induced proliferation of endothelial progenitor cells via inhibition of HIF-1a-mediated MAPK and AKT signaling. Exp Ther Med 13:1017-1024 (2017). WB ; Human . Read more (PubMed: 28450935) »
  • Reeder JE  et al. HIV Tat controls RNA Polymerase II and the epigenetic landscape to transcriptionally reprogram target immune cells. Elife 4:N/A (2015). WB, ChIP . Read more (PubMed: 26488441) »

See all 3 Publications for this product

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Most antibodies will work fine with either BSA or milk.

When testing our antibodies, our lab uses 5% BSA as a blocking reagent, so I recommend this.

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