Anti-KMT2G / hSET1B抗体(ab113984)


  • 产品名称Anti-KMT2G / hSET1B抗体
    参阅全部 KMT2G / hSET1B 一抗
  • 描述
    兔多克隆抗体to KMT2G / hSET1B
  • 经测试应用适用于: IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Cow, Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide corresponding to Human KMT2G/ hSET1B aa 350-400.
    Database link: 23067

  • 阳性对照
    • HeLa cell lysate


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab113984 is affinity purified using an epitope specific to KMT2G / hSET1B immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域



Our Abpromise guarantee covers the use of ab113984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.

ab113984 has not performed satisfactorily when used for WB of KMT2G / hSET1B in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of KMT2G / hSET1B.

IP Use at 10 µg/mg of lysate.


  • 功能Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1A suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression. Specifically tri-methylates 'Lys-4' of histone H3 in vitro.
  • 序列相似性Contains 1 post-SET domain.
    Contains 1 RRM (RNA recognition motif) domain.
    Contains 1 SET domain.
  • 细胞定位Nucleus speckle. Chromosome. Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1A, suggesting that SETD1A and SET1B each bind to a unique set of target genes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ20803 antibody
    • Histone-lysine N-methyltransferase SETD1B antibody
    • hSET1B antibody
    • KIAA1076 antibody
    • KMT2G antibody
    • Lysine N-methyltransferase 2G antibody
    • SET domain containing 1B antibody
    • SET domain-containing protein 1B antibody
    • SET1B antibody
    • SET1B_HUMAN antibody
    • SETD1B antibody
    see all

Anti-KMT2G / hSET1B antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling KMT2G / hSET1B with ab113984 at 1/1000 (1µg/ml). Detection: DAB.
  • ab113984 used at 10 µg/mg of lysate staining KMT2G / hSET1B in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) by immunoprecipitation. For blotting immunoprecipitated KMT2G / hSET1B, ab113984 was used at 1 µg/ml.

    Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-KMT2G / hSET1B antibody (ab113984)参考文献

ab113984 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Permeabilization Yes - 0.5% TritonX100 in PBS
Specification HeLa
Fixative Paraformaldehyde

Dr. Kirk McManus

Verified customer

提交于 Jun 19 2015