Anti-SETD1B抗体(ab113984)
Key features and details
- Rabbit polyclonal to SETD1B
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SETD1B抗体
参阅全部 SETD1B 一抗 -
描述
兔多克隆抗体to SETD1B -
宿主
Rabbit -
经测试应用
适用于: IHC-P, IPmore details -
种属反应性
与反应: Human
预测可用于: Rabbit, Cow, Chimpanzee, Gorilla -
免疫原
Synthetic peptide corresponding to Human SETD1B aa 350-400.
Database link: 23067 -
阳性对照
- HeLa cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab113984 is affinity purified using an epitope specific to SETD1B immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab113984于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
Use at 10 µg/mg of lysate.
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说明 |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Use at 10 µg/mg of lysate. |
靶标
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功能
Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1A suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression. Specifically tri-methylates 'Lys-4' of histone H3 in vitro. -
序列相似性
Contains 1 post-SET domain.
Contains 1 RRM (RNA recognition motif) domain.
Contains 1 SET domain. -
细胞定位
Nucleus speckle. Chromosome. Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1A, suggesting that SETD1A and SET1B each bind to a unique set of target genes. - Information by UniProt
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数据库链接
- Entrez Gene: 23067 Human
- Omim: 611055 Human
- SwissProt: Q9UPS6 Human
- Unigene: 507122 Human
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别名
- FLJ20803 antibody
- Histone-lysine N-methyltransferase SETD1B antibody
- hSET1B antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling SETD1B with ab113984 at 1/1000 (1µg/ml). Detection: DAB.
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ab113984 used at 10 µg/mg of lysate staining SETD1B in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) by immunoprecipitation. For blotting immunoprecipitated SETD1B, ab113984 was used at 1 µg/ml.
Detection: Chemiluminescence with an exposure time of 3 minutes.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab113984 被引用在 3 文献中.
- Lindner P et al. EMT transcription factor ZEB1 alters the epigenetic landscape of colorectal cancer cells. Cell Death Dis 11:147 (2020). PubMed: 32094334
- Yang P et al. SET domain containing 1B gene is mutated in primary hepatic neuroendocrine tumors. Int J Cancer 145:2986-2995 (2019). PubMed: 30977120
- Chen D et al. High-level SETD1B gene expression is associated with unfavorable prognosis in hepatocellular carcinoma. Mol Med Rep 19:1587-1594 (2019). PubMed: 30628696