Anti-KMT1E / SETDB1抗体- ChIP Grade (ab12317)


  • 产品名称Anti-KMT1E / SETDB1抗体- ChIP Grade
    参阅全部 KMT1E / SETDB1 一抗
  • 描述
    兔多克隆抗体to KMT1E / SETDB1 - ChIP Grade
  • 经测试应用适用于: ChIP, ICC/IF, WB, IHC-P, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Immunogen was a synthetic peptide, which represented a portion of human SET domain, bifurcated 1 encoded within exon 19 (LocusLink ID 9869).

  • 阳性对照
    • 293T cells.
  • 常规说明Centrifuge tube to remove product from lid.



Our Abpromise guarantee covers the use of ab12317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 21921037
WB 1/1000 - 1/10000. Predicted molecular weight: 155 kDa.
IHC-P Use at an assay dependent concentration.
IP Use a concentration of 1 - 4 mg/ml.


  • 功能Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins.
  • 组织特异性Widely expressed. High expression in testis.
  • 序列相似性Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
    Contains 1 MBD (methyl-CpG-binding) domain.
    Contains 1 post-SET domain.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
    Contains 2 Tudor domains.
  • 结构域The pre-SET, SET and post-SET domains are all required for methyltransferase activity. The 347-amino-acid insertion in the SET domain has no effect on the catalytic activity.
    Isoform 2 lacks all domains required for histone methyltransferase activity.
  • 细胞定位Nucleus. Chromosome. Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AU022152 antibody
    • EC antibody
    • ERG-associated protein with SET domain antibody
    • ESET antibody
    • H3 K9 HMTase 4 antibody
    • H3-K9-HMTase 4 antibody
    • H3-K9-HMTase4 antibody
    • Histone H3 K9 methyltransferase 4 antibody
    • Histone H3-K9 methyltransferase 4 antibody
    • Histone-lysine N-methyltransferase SETDB1 antibody
    • KG1T antibody
    • KIAA0067 antibody
    • KMT1E antibody
    • Lysine N-methyltransferase 1E antibody
    • MGC90670 antibody
    • mKIAA0067 antibody
    • SET domain bifurcated 1 antibody
    • Set domain protein, bifurcated, 1 antibody
    • SETB1_HUMAN antibody
    • Setdb1 antibody
    see all

Anti-KMT1E / SETDB1 antibody - ChIP Grade 图像

  • Whole cell lysate (2 mg) from 293T cells that were mock transfected (1 and 2) or transfected with a SETDB1 expression construct (3 and 4). Rabbit anti-SETDB1 antibodies; ab12317 (2 and 4) and a competitor's antiserum (1 and 3) were used at 1 mcg/mg lysate for IP. ab12317 was used at 0.2 mcg/ml for Western Blot. Detection: Chemiluminescence with exposure times of 1 to 5 minutes.

  • Predicted band size : 155 kDa
  • ab12317 (4ug/ml) staining KMT1E in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of KMT1E in nuclear compartments of the germinal epithelium.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-KMT1E / SETDB1 antibody - ChIP Grade (ab12317)参考文献

This product has been referenced in:
  • Hatanaka Y  et al. Histone chaperone CAF-1 mediates repressive histone modifications to protect preimplantation mouse embryos from endogenous retrotransposons. Proc Natl Acad Sci U S A 112:14641-6 (2015). ICC/IF ; Mouse . Read more (PubMed: 26546670) »
  • Calderon MR  et al. Ligand-dependent corepressor contributes to transcriptional repression by C2H2 zinc-finger transcription factor ZBRK1 through association with KRAB-associated protein-1. Nucleic Acids Res 42:7012-27 (2014). ChIP . Read more (PubMed: 24829459) »

See all 14 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (brain)
Permeabilization Yes - 0.5% Triton
Specification brain
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative Paraformaldehyde

Abcam user community

Verified customer

提交于 Sep 01 2015

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C
Sample Mouse Tissue sections (Ovary section)
Specification Ovary section
Permeabilization Yes - Trition
Fixative Paraformaldehyde

Abcam user community

Verified customer

提交于 Jan 30 2015

Thank you for taking time to answer the additional questions. I am sorry to hear this antibody is not providing satisfactory results.
The details provided will enable us to investigate this case and will provide us with vital information for monit...

Read More

Thanks for your enquiry. There are two western blots on the datasheet for ab12317. I believe you are referring to the bottom image. I think there is a mistake in the annotation for this blot. 'Mock transfected' should refer to lanes 3 and 4 of this blo...

Read More

Thank you for your enquiry. The predicted molecular weight of SETDB1 is 155kDa, based on the amino acid composition of the protein. It should be noted however that this protein is known to be phophorylated, as indicated on the Swissprot data page entry...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP on chip
Sample Mouse Cell lysate - nuclear (mouse ES cells)
Specification mouse ES cells
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Detection step Microarray
Positive control Competitor Antibody

Dr. Steve Bilodeau

Verified customer

提交于 Oct 21 2009

The precise sequence used to generate ab12317 is proprietary. The epitope recognized by ab12317 is found between residues 1125 and 1175 of SwissProt entry Q15047 (