Anti-KMT1E / SETDB1抗体[5H6A12] (ab107225)


  • 产品名称Anti-KMT1E / SETDB1抗体[5H6A12]
    参阅全部 KMT1E / SETDB1 一抗
  • 描述
    小鼠单克隆抗体[5H6A12] to KMT1E / SETDB1
  • 经测试应用适用于: Flow Cyt, WB, ICC/IF, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human, Monkey
  • 免疫原

    Purified recombinant fragment of human SETDB1 expressed in E. Coli.

  • 阳性对照
    • MCF-7,T47D, HEK293, JURKAT, NIH/3T3, F9, RAW246.7 and COS-7 cell lysates.



Our Abpromise guarantee covers the use of ab107225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/500 - 1/2000. Predicted molecular weight: 180 kDa.
ICC/IF 1/200 - 1/1000.
ELISA 1/10000.


  • 功能Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins.
  • 组织特异性Widely expressed. High expression in testis.
  • 序列相似性Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
    Contains 1 MBD (methyl-CpG-binding) domain.
    Contains 1 post-SET domain.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
    Contains 2 Tudor domains.
  • 结构域The pre-SET, SET and post-SET domains are all required for methyltransferase activity. The 347-amino-acid insertion in the SET domain has no effect on the catalytic activity.
    Isoform 2 lacks all domains required for histone methyltransferase activity.
  • 细胞定位Nucleus. Chromosome. Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AU022152 antibody
    • EC antibody
    • ERG-associated protein with SET domain antibody
    • ESET antibody
    • H3 K9 HMTase 4 antibody
    • H3-K9-HMTase 4 antibody
    • H3-K9-HMTase4 antibody
    • Histone H3 K9 methyltransferase 4 antibody
    • Histone H3-K9 methyltransferase 4 antibody
    • Histone-lysine N-methyltransferase SETDB1 antibody
    • KG1T antibody
    • KIAA0067 antibody
    • KMT1E antibody
    • Lysine N-methyltransferase 1E antibody
    • MGC90670 antibody
    • mKIAA0067 antibody
    • SET domain bifurcated 1 antibody
    • Set domain protein, bifurcated, 1 antibody
    • SETB1_HUMAN antibody
    • Setdb1 antibody
    see all

Anti-KMT1E / SETDB1 antibody [5H6A12] 图像

  • All lanes : Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225) at 1/500 dilution

    Lane 1 : MCF-7
    Lane 2 : T47D
    Lane 3 : HEK293
    Lane 4 : JURKAT
    Lane 5 : NIH/3T3
    Lane 6 : F9
    Lane 7 : RAW246.7
    Lane 8 : COS-7

    Predicted band size : 180 kDa
  • Immunofluoresence detection of SETDB1 in LOVO cells using ab107225 (green). Actin fillaments have been labeled with Alexa Fluor-555 phalloidin (red).
  • Overlay histogram showing Ramos cells stained with ab107225 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab107225, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225)参考文献

ab107225 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293T and mouse ESC)
Gel Running Conditions Reduced Denaturing (4-15% TGX BioRad)
Loading amount 200000 cells
Specification HEK293T and mouse ESC
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C

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提交于 Jan 05 2016